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题名: Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo
作者: Zhang, Ying1, 2;  Wang, Wei1;  Xie, Yubing1;  Yu, Weiting1;  Lv, Guojun1, 2;  Guo, Xin1;  Xiong, Ying1;  Ma, Xiaojun1
关键词: microencapsulation ;  recombinant cell ;  cell growth ;  endostatin production ;  stability ;  in vivo
刊名: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
发表日期: 2008
卷: 84B, 期:1, 页:79-88
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Microencapsulation of recombinant cells secreting endostatin offers a promising approach to tumor gene therapy in which therapeutic protein is delivered in a sustainable and long-term fashion by encapsulated recombinant cells. However, the studies of cell growth and protein production in vivo are very limited. In this study, the effects of microencapsulation parameters on in vivo cell growth, endostatin production, and microcapsule stability after implantation in the peritoneal cavity of mice were for the first time investigated. Microcapsules with liquid core reached higher cell density and endostatin production at day 18 than microcapsules with solid core. There was no significant difference in stability whether the core of the microcapsulle was solid or liquid. Decrease in microcapsule size increased the stability of microcapsule. The microcapsules kept intact in the peritoneal cavity of mice after 36 days of implantation when the microcapsules size was 240 mu m in diameter, which gave rise to high endostatin production as well. The optimized microencapsulation conditions for in vivo implantation are liquid core and 240 mu m in diameter. This study provides useful information for antiangiogenic gene therapy to tumors using microencapsulated recombinant cells. (c) 2007 Wiley Periodicals, Inc.
关键词[WOS]: SOMATIC GENE-THERAPY ;  HUMAN FACTOR-IX ;  ALGINATE MICROCAPSULES ;  MAMMALIAN-CELLS ;  TECHNOLOGY ;  DELIVERY ;  ENCAPSULATION ;  FIBROBLASTS ;  CHEMISTRY ;  MYOBLASTS
语种: 英语
WOS记录号: WOS:000251802900010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140863
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Zhang, Ying,Wang, Wei,Xie, Yubing,et al. Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2008,84B(1):79-88.
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