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题名: Characterization of multi-phosphopeptides by mu HPLC-ESI-MS/MS with a alkaline phosphatase treatment
作者: Wang, Hui1, 2;  Duan, Jicheng1, 2;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Weibing1;  Zhang, Yukui1
关键词: alkaline phosphatase ;  dephosphorylation ;  multi-phosphopeptide ;  IHPLC-ESI-MS/MS
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2008-02-01
DOI: 10.1002/jssc.200700445
卷: 31, 期:3, 页:480-487
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The detection of phosphopeptides, especially multi-phosphopeptides, by tandem electrospray ionization mass spectrometry (ESI-MS/MS) is a great challenge due to their low abundance and the poor ionization efficiency of samples. In our recent study, a strategy was proposed for the analysis of trace multi-phosphopeptides which combined selective enrichment of phosphorylated peptides by TiO2 and dephosphorylation by alkaline phosphatase (AP). After separation by mu HPLC, the profiles of enriched peptides before and after AP treatment were compared, and the additional peaks appearing in the latter case hinted at the existence of multi-phosphopeptides. Subsequently, an incomplete dephosphorylation reaction was performed to partially remove the phosphate groups so that the phosphorylation sites of the multi-phosphopeptides might be estimated. Through analysis of the digests of beta-casein and extracted proteins of bovine milk, more information on the multi-phosphopeptides was obtained by mu HPLC-ESI-MS/MS than that obtained without AP treatment, which demonstrated that such a strategy might supply some potential information about trace multi-phosphopeptides lost in shotgun analysis.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  PROTEIN-PHOSPHORYLATION ;  MALDI MS ;  ENRICHMENT ;  IDENTIFICATION ;  PEPTIDES ;  PHOSPHOPROTEINS ;  NANOPARTICLES ;  PROTEOMICS
语种: 英语
WOS记录号: WOS:000253689700006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140870
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Wang, Hui,Duan, Jicheng,Zhang, Lihua,et al. Characterization of multi-phosphopeptides by mu HPLC-ESI-MS/MS with a alkaline phosphatase treatment[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(3):480-487.
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