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题名: Direct electron transfer of horseradish peroxidase and its biosensor based on gold nanoparticles/chitosan/ITO modified electrode
作者: Xiang, Cuili2;  Zou, Yongjin2;  Sun, Li-Xian1;  Xu, Fen
关键词: chitosan ;  direct electron transfer ;  gold nanoparticles ;  horseradish peroxidase ;  ITO electrode
刊名: ANALYTICAL LETTERS
发表日期: 2008
DOI: 10.1080/00032710802238150
卷: 41, 期:12, 页:2224-2236
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Gold nanoparticles/chitosan modified indium tin oxide (ITO) coated glass electrode was prepared by a simple layer-by-layer self-assembly technique. Horseradish peroxidase (HRP) was successfully immobilized on the modified electrode by electrostatic adsorption. Direct electrochemistry and electrocatalysis of HRP were investigated. The composite film showed an obvious promotion for the direct electron transfer between HRP and the underlying electrode. The immobilized HRP exhibited an excellent electrocatalytic activity toward the reduction of H(2)O(2). The catalysis currents increased linearly to the H(2)O(2) concentration in a wide range of 2.0 x 10(-5) to 6.5 x 10(-3) M with a low detection limit of 3.5 x 10(-6) M. The calculated apparent Michaelis-Menten constant (K(m)(app)) was 4.43mM.
关键词[WOS]: DIRECT ELECTROCHEMISTRY ;  HYDROGEN-PEROXIDE ;  CYTOCHROME-C ;  ELECTROCATALYSIS ;  NANOCOMPOSITE ;  IMMOBILIZATION ;  VOLTAMMETRY ;  PROTEINS ;  CHITOSAN ;  ENZYMES
语种: 英语
WOS记录号: WOS:000258487500009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140874
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Mat & Thermochem Lab, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Xiang, Cuili,Zou, Yongjin,Sun, Li-Xian,et al. Direct electron transfer of horseradish peroxidase and its biosensor based on gold nanoparticles/chitosan/ITO modified electrode[J]. ANALYTICAL LETTERS,2008,41(12):2224-2236.
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