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题名: Studies on Pathway of Producing Terephthalic Acid by Microorganism Coordinated Catalysis
作者: Sang Ping1, 2;  Tian Jing1, 2;  Gao Peng2;  Xu Guo-Wang2
关键词: Terephthalic acid ;  high performance liquid chromatography ;  biocatalysis ;  comamonas testosteroni ;  stenotrophomonas maltophilia
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2008-08-01
卷: 36, 期:8, 页:1024-1028
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A method was developed for the determination of terephthalic acid, p-tolualdehyde, p-methylbenzyl alcohol and p-toluic acid by HPLC. Hypersil SAX column was used. The mobile phase was 2.5 mol/L NH4H2PO4 containing 10 % acetonitrile, pH was 4. 32. The flow rate was 0. 8 mL/min, the temperature of column was 30 T and detected wavelength was 254 nm. In this study, each component was completely separated and determined in 7 min. The recovery accorded with detecting requirement. The method Was used to analyze the main metabolites in fermentation broth at different ferment times, which were produced at the process of biocatalysis producing terephthalic acid by Stenotrophomonas maltophilia and Comamonas testosteroni. The metabolites in cell including organic acids, amino acids, fatty acids and sugars were detected by GC-MS. According to the analytical results of HPLC and GC-MS, the pathway of producing terephthalic acid by stenotrophomonas maltophilia and comamonas testosteroni coordinated catalysis was discussed.
关键词[WOS]: TOLUENE
语种: 英语
WOS记录号: WOS:000260136800003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140887
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Polytech Univ, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Sang Ping,Tian Jing,Gao Peng,et al. Studies on Pathway of Producing Terephthalic Acid by Microorganism Coordinated Catalysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(8):1024-1028.
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