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Studies on Pathway of Producing Terephthalic Acid by Microorganism Coordinated Catalysis
Sang Ping1,2; Tian Jing1,2; Gao Peng2; Xu Guo-Wang2
关键词Terephthalic Acid High Performance Liquid Chromatography Biocatalysis Comamonas Testosteroni Stenotrophomonas Maltophilia
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2008-08-01
36期:8页:1024-1028
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TOLUENE
英文摘要A method was developed for the determination of terephthalic acid, p-tolualdehyde, p-methylbenzyl alcohol and p-toluic acid by HPLC. Hypersil SAX column was used. The mobile phase was 2.5 mol/L NH4H2PO4 containing 10 % acetonitrile, pH was 4. 32. The flow rate was 0. 8 mL/min, the temperature of column was 30 T and detected wavelength was 254 nm. In this study, each component was completely separated and determined in 7 min. The recovery accorded with detecting requirement. The method Was used to analyze the main metabolites in fermentation broth at different ferment times, which were produced at the process of biocatalysis producing terephthalic acid by Stenotrophomonas maltophilia and Comamonas testosteroni. The metabolites in cell including organic acids, amino acids, fatty acids and sugars were detected by GC-MS. According to the analytical results of HPLC and GC-MS, the pathway of producing terephthalic acid by stenotrophomonas maltophilia and comamonas testosteroni coordinated catalysis was discussed.
语种英语
WOS记录号WOS:000260136800003
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140887
专题中国科学院大连化学物理研究所
作者单位1.Dalian Polytech Univ, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Sang Ping,Tian Jing,Gao Peng,et al. Studies on Pathway of Producing Terephthalic Acid by Microorganism Coordinated Catalysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(8):1024-1028.
APA Sang Ping,Tian Jing,Gao Peng,&Xu Guo-Wang.(2008).Studies on Pathway of Producing Terephthalic Acid by Microorganism Coordinated Catalysis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(8),1024-1028.
MLA Sang Ping,et al."Studies on Pathway of Producing Terephthalic Acid by Microorganism Coordinated Catalysis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.8(2008):1024-1028.
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