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题名: Simple, fast and high-throughput single-cell analysis on PDMS microfluidic chips
作者: Yu, Linfen1, 2;  Huang, Huaiqing1, 2;  Dong, Xiuling3;  Wu, Dapeng1, 2;  Qin, Jinhua1;  Lin, Bingcheng1
关键词: K562 cells ;  Microfluidic chip ;  Single-cell analysis
刊名: ELECTROPHORESIS
发表日期: 2008-12-01
DOI: 10.1002/elps.200800331
卷: 29, 期:24, 页:5055-5060
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: This paper demonstrated the chemical analysis of single cell on a cross PDMS microfluidic chip in a simple, fast, and high-throughput mode. The pre-stained cells were sequentially loaded into the cross section by hydrodynamic force, lysed by 0.2% SDS and subsequently the lysates were detected by LIF. Each cell can be lysed within 500 ms due to its high concentration of SDS at cross section resulted from the absence of electro-osmosis after surface coating in microchannel. The reliability and quality of the analysis was confirmed by analysis of glutathione and rhodamine 123 in single K562 cells. In each run, approximately 100 cells could be analyzed in about 10 min, which demonstrated the comparatively high throughput. The proposed microfluidic method is simple, fast, and high throughput, which might be of significance in identifying the biological molecules involved in fast biochemical processes and studying heterogenous cells.
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ;  CHEMICAL CYTOMETRY ;  HUMAN ERYTHROCYTES ;  MASS-SPECTROMETRY ;  BACTERIAL-SPORES ;  MAMMALIAN-CELLS ;  FLUORESCENCE ;  GLUTATHIONE ;  SEPARATION ;  INJECTION
语种: 英语
WOS记录号: WOS:000262565700030
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140942
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Liaoning, Peoples R China
2.Chinese Acad Sci, Grad Sch, Dalian 116023, Liaoning, Peoples R China
3.Dalian Third Municipal Hosp, Dalian, Peoples R China

Recommended Citation:
Yu, Linfen,Huang, Huaiqing,Dong, Xiuling,et al. Simple, fast and high-throughput single-cell analysis on PDMS microfluidic chips[J]. ELECTROPHORESIS,2008,29(24):5055-5060.
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