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Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid
Cao, Qing1; Quan, Li1; He, Chiyang2; Li, Na1; Li, Kean1; Liu, Feng1
关键词Horseradish Peroxidase Enzyme Activity Partition Ionic Liquid Aqueous Biphasic System
刊名TALANTA
2008-10-15
DOI10.1016/j.talanta.2008.05.055
77期:1页:160-165
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ANTARCTICA LIPASE-B ; 2-PHASE SYSTEM ; SOLVENT-EXTRACTION ; CROWN-ETHER ; SEPARATION ; WATER ; SALT ; SELECTIVITY ; STABILITY ; MEDIA
英文摘要Enzyme activity and partition behavior in aqueous biphasic systems (ABSs) consisting of ionic liquid (IL) and salt (IL-ABSs) were investigated to increase our understanding of IL-ABSs and shed light on their application potential as enzyme extraction system. With horseradish peroxidase (HRP) as the model enzyme, several effects of alkylimidazolium chloride-K2HPO4 ABSs on activity and partition behavior of enzyme were studied including alkyl chain length of ILs and concentrations of each component. High lyotropic ILs (1-butyl-3-methylimidazolium and 1-ethyl-3-methylimidazolium chloride) and adequate water content (>40%) were both essential for the activity maintenance of HRP in IL-ABS. 1-Butyl-3-methylimidazolium chloride ([C(4)mim]Cl) was found to be an appropriate IL for phase forming and HRP activity retaining. After optimization of phase condition, about 80% HRP amount was distributed in the IL-rich upper phase, and greater than 90% enzyme activity was obtained. Moreover, compared with the commonly used polymer-based ABSs, this [C(4)mim]Cl-ABS has a much lower viscosity, which is very beneficial to the experimental operation. Therefore, the tested IL-ABS could be considered as a potential enzyme extraction system. (C) 2008 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000260290200024
引用统计
被引频次:56[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140956
专题中国科学院大连化学物理研究所
作者单位1.Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China
2.Anqing Normal Coll, Dept Chem, Anqing 246011, Peoples R China
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GB/T 7714
Cao, Qing,Quan, Li,He, Chiyang,et al. Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid[J]. TALANTA,2008,77(1):160-165.
APA Cao, Qing,Quan, Li,He, Chiyang,Li, Na,Li, Kean,&Liu, Feng.(2008).Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid.TALANTA,77(1),160-165.
MLA Cao, Qing,et al."Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid".TALANTA 77.1(2008):160-165.
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