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题名: Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid
作者: Cao, Qing1;  Quan, Li1;  He, Chiyang2;  Li, Na1;  Li, Kean1;  Liu, Feng1
关键词: Horseradish peroxidase ;  Enzyme activity ;  Partition ;  Ionic liquid ;  Aqueous biphasic system
刊名: TALANTA
发表日期: 2008-10-15
DOI: 10.1016/j.talanta.2008.05.055
卷: 77, 期:1, 页:160-165
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Enzyme activity and partition behavior in aqueous biphasic systems (ABSs) consisting of ionic liquid (IL) and salt (IL-ABSs) were investigated to increase our understanding of IL-ABSs and shed light on their application potential as enzyme extraction system. With horseradish peroxidase (HRP) as the model enzyme, several effects of alkylimidazolium chloride-K2HPO4 ABSs on activity and partition behavior of enzyme were studied including alkyl chain length of ILs and concentrations of each component. High lyotropic ILs (1-butyl-3-methylimidazolium and 1-ethyl-3-methylimidazolium chloride) and adequate water content (>40%) were both essential for the activity maintenance of HRP in IL-ABS. 1-Butyl-3-methylimidazolium chloride ([C(4)mim]Cl) was found to be an appropriate IL for phase forming and HRP activity retaining. After optimization of phase condition, about 80% HRP amount was distributed in the IL-rich upper phase, and greater than 90% enzyme activity was obtained. Moreover, compared with the commonly used polymer-based ABSs, this [C(4)mim]Cl-ABS has a much lower viscosity, which is very beneficial to the experimental operation. Therefore, the tested IL-ABS could be considered as a potential enzyme extraction system. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: ANTARCTICA LIPASE-B ;  2-PHASE SYSTEM ;  SOLVENT-EXTRACTION ;  CROWN-ETHER ;  SEPARATION ;  WATER ;  SALT ;  SELECTIVITY ;  STABILITY ;  MEDIA
语种: 英语
WOS记录号: WOS:000260290200024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140956
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China
2.Anqing Normal Coll, Dept Chem, Anqing 246011, Peoples R China

Recommended Citation:
Cao, Qing,Quan, Li,He, Chiyang,et al. Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid[J]. TALANTA,2008,77(1):160-165.
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