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Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics
Tian, Jing2; Shi, Chunyun2; Gao, Peng; Yuan, Kailong; Yang, Dawei2; Lu, Xin; Xu, Guowang1
关键词Metabolomics E. Coli Phenotype Gc-fid Gc-ms
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2008-08-15
DOI10.1016/j.jchromb.2008.06.031
871期:2页:220-226
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SOLID-PHASE MICROEXTRACTION ; SYSTEMS BIOLOGY ; FUNCTIONAL GENOMICS ; HEADSPACE-GC ; SPECTROMETRY ; NETWORKS ; STRATEGY ; ENZYMES ; GROWTH ; WATER
英文摘要Two mutants of E call with deletion of sdhAB and ackA-pta genes respectively and their wild-type strains were Subjected to gas chromatography-flame-ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) metabolomics analysis. Intracellular metabolites of the three strains were profiled by GC-FID firstly. Methodological evaluation of the employed platform indicated that the limit of detection ranges were from 0.2 to 12.5 ng for some representative metabolites and the corresponding recoveries were varied from 68.7 to 122.7%. Secondly, multivariable data analysis was applied to the acquired data sets. As expected, the three phenotypes could be easily differentiated, and the perturbed metabolite pools in the genetically modified strains Were screened. Lastly, the metabolites playing key roles in the differentiation were further identified by GC-MS. It was confirmed that succinic acid and aspartic acid were similarly affected in the modified strains. But proline content was altered contrarily. Additionally, deletion of sdhAB gene also affected the growth property of relevant mutant greatly. The potential mechanism was postulated accordingly. (C) 2008 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000259130400009
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被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/140962
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Polytech Univ, Dept Modern Technol, Dalian 116034, Peoples R China
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Tian, Jing,Shi, Chunyun,Gao, Peng,et al. Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,871(2):220-226.
APA Tian, Jing.,Shi, Chunyun.,Gao, Peng.,Yuan, Kailong.,Yang, Dawei.,...&Xu, Guowang.(2008).Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,871(2),220-226.
MLA Tian, Jing,et al."Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 871.2(2008):220-226.
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