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题名: Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics
作者: Tian, Jing2;  Shi, Chunyun2;  Gao, Peng;  Yuan, Kailong;  Yang, Dawei2;  Lu, Xin;  Xu, Guowang1
关键词: metabolomics ;  E. coli ;  phenotype ;  GC-FID ;  GC-MS
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2008-08-15
DOI: 10.1016/j.jchromb.2008.06.031
卷: 871, 期:2, 页:220-226
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Two mutants of E call with deletion of sdhAB and ackA-pta genes respectively and their wild-type strains were Subjected to gas chromatography-flame-ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) metabolomics analysis. Intracellular metabolites of the three strains were profiled by GC-FID firstly. Methodological evaluation of the employed platform indicated that the limit of detection ranges were from 0.2 to 12.5 ng for some representative metabolites and the corresponding recoveries were varied from 68.7 to 122.7%. Secondly, multivariable data analysis was applied to the acquired data sets. As expected, the three phenotypes could be easily differentiated, and the perturbed metabolite pools in the genetically modified strains Were screened. Lastly, the metabolites playing key roles in the differentiation were further identified by GC-MS. It was confirmed that succinic acid and aspartic acid were similarly affected in the modified strains. But proline content was altered contrarily. Additionally, deletion of sdhAB gene also affected the growth property of relevant mutant greatly. The potential mechanism was postulated accordingly. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: SOLID-PHASE MICROEXTRACTION ;  SYSTEMS BIOLOGY ;  FUNCTIONAL GENOMICS ;  HEADSPACE-GC ;  SPECTROMETRY ;  NETWORKS ;  STRATEGY ;  ENZYMES ;  GROWTH ;  WATER
语种: 英语
WOS记录号: WOS:000259130400009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140962
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Polytech Univ, Dept Modern Technol, Dalian 116034, Peoples R China

Recommended Citation:
Tian, Jing,Shi, Chunyun,Gao, Peng,et al. Phenotype differentiation of three E-coli strains by GC-FID and GC-MS based metabolomics[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,871(2):220-226.
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