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题名: The analysis of chemotherapy resistance in human lung cancer cell line with microchip-based system
作者: Ying-Yan, Wang2;  Tao, Wang3;  Xin, Liu1;  Hong-Wei, Gai4;  Bing-Cheng, Lin1;  Qi, Wang5
关键词: GRP78 ;  microchip-based system ;  immunofluorescence ;  electrophoresis ;  VP-16 ;  chemotherapy resistance
刊名: BIOMEDICAL MICRODEVICES
发表日期: 2008-06-01
DOI: 10.1007/s10544-007-9152-5
卷: 10, 期:3, 页:429-435
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Nanoscience & Nanotechnology
研究领域[WOS]: Engineering ;  Science & Technology - Other Topics
英文摘要: Microchip-based systems have many desirable characteristics and can be used in much cellular biochemical analysis. Glucose-regulated protein 78 (GRP78), an endoplasmic reticulum chaperone, has a critical role in chemotherapy resistance of some cancers. This work aimed at analyzing the correlation between the expression of GRP78 and an anticancer drug, topoisomerase II inhibitor-VP-16, in human lung cancer cell line NCI-H460 using this microchip-based system. The cells were cultured on a PDMS chip, the expression of GRP78 at both protein and mRNA levels for the cells under the condition with or without the induction of A23187 were assayed by immunofluorescence and chip electrophoresis, respectively. Then the cells were treated by VP-16, percentages of apoptosis and the cycle distributions of the cells were detected by flow cytometry. The cells cultured on the PDMS attached and spread well to micro-channels with high viability. Compared with the non-induced cells, the expression of GRP78 at both protein and mRNA levels for the A23187-induced cells were increased greatly. After treatment by VP-16, the percentage of apoptotic cells decreased nearly threefold for the A23187-induced cells in contrast to the non-induced cells (13.15 +/- 3.84% versus 34.03 +/- 11.45%), and the cells distributed in S phase reduced dramatically (11.96 +/- 1.27% versus 20.76 +/- 3.05%) whereas in G(1) phase increased greatly (74.16 +/- 0.95% versus 57.06 +/- 4%). GRP78 is correlated to the resistance to VP-16 in human lung cancer cell line. The microchip-based system has the potential application and feasibility for cell culture and its functional research.
关键词[WOS]: BIOCHEMICAL-ANALYSIS SYSTEMS ;  MICROFLUIDIC SYSTEMS ;  STRESS-RESPONSE ;  EXPRESSION ;  INTEGRATION ;  TRENDS ;  DEVICE ;  VP-16
语种: 英语
WOS记录号: WOS:000254900300012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/140970
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
2.Dal Med Univ, Lab Ctr Diagnost, Dalian 116023, Liaoning, Peoples R China
3.Dalian Univ, Dept Resp Med, Affiliated Zhongshan Hosp, Dalian 116001, Liaoning, Peoples R China
4.Hunan Univ, Ctr Biomed Engn, Changsha 410082, Hunan, Peoples R China
5.Dalian Med Univ, Dept Resp Med, Hosp 2, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Ying-Yan, Wang,Tao, Wang,Xin, Liu,et al. The analysis of chemotherapy resistance in human lung cancer cell line with microchip-based system[J]. BIOMEDICAL MICRODEVICES,2008,10(3):429-435.
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