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题名: Biological fingerprinting analysis of interaction between taxoids in Taxus and microtubule protein by microdialysis coupled, with high-performance liquid chromatography/mass spectrometry for screening antimicrotubule agents
作者: Lei Xiao-yuan1;  Kong Liang2;  Su Xing-ye1;  Guo Ming3;  Zou Han-fa1
关键词: microdialysis/high-performance liquid chromatography(HPLC) ;  biological fingerprinting analysis ;  taxoids ;  microtubule ;  Taxus
刊名: CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
发表日期: 2008-07-01
卷: 24, 期:4, 页:411-419
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Some natural products, such as traditional Chinese medicines(TCMs), contain compounds with anticancer activity and have attracted a great interest in recent years as alternative anticancer therapies. A quick and convenient assay for screening antimicrotubule compounds in which in vitro microdialysis/high-performance liquid chromatography (HPLC) is used to monitor the binding of the compounds extracted from TCM Taxus cuspidata Siebold & Zucc(Taxus) to microtubules is reported. It was observed that the extract of Taxus contains at least five compounds which have affinity interaction with microtubules by biological fingerprinting analysis, and they were identified as the taxoids of taxol, baccatin III, 10-deacetylbaccatin III(10-DAB), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT) based on the comparison of their high-performance liquid chromatographic/mass spectrometric and UV spectra with those of the standard samples, both assembly-promoting and disassembly-inhibiting characteristics of those compounds were evaluated. It was observed that baccatin Ill and 10-DAB bound to microtubules and the binding degrees were influenced by GTP. Competitive binding behavior of taxol with other four taxoids to microtubules was also investigated.
关键词[WOS]: TRADITIONAL CHINESE MEDICINES ;  JAPANESE YEW ;  BIOACTIVE COMPOUNDS ;  TUBULIN ;  CUSPIDATA ;  DRUGS ;  CHEMOTHERAPY ;  MODULATION ;  INVITRO ;  TARGET
语种: 英语
WOS记录号: WOS:000258446000006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141005
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China
3.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China

Recommended Citation:
Lei Xiao-yuan,Kong Liang,Su Xing-ye,et al. Biological fingerprinting analysis of interaction between taxoids in Taxus and microtubule protein by microdialysis coupled, with high-performance liquid chromatography/mass spectrometry for screening antimicrotubule agents[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2008,24(4):411-419.
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