中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Retention time mass-charge ratio pairs for label-free differential analysis of peptides
作者: Li Xin1;  Xu Song-Yun1;  Zhang Yu1;  Zou Han-Fa1
关键词: label-free ;  liquid chromatography-mass spectrometry ;  quantitative proteomics ;  peptidome
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2008-07-01
卷: 36, 期:7, 页:867-873
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A simple LC-MS based methodology is presented that allows relative changes in abundance of peptides In highly complex mixtures to he determined. Proposed method involves signal extraction, generation of features, alignment of corresponding features across different replicates/samples, and monitoring fold changes in relative abundance among different conditions without requiring the use of isotopic or metabolic labeling strategies. Utilizing a nano-flow chromatographic separation system along with the high mass resolution and mass accuracy of all electrospray-ionization (ESI) ion-trap mass spectrometer, the quantitative comparison of thousands of ions emanating front identically prepared control and experimental samples can be made. Using this configuration, the change in relative abundance of a small number of ions between the two conditions solely can I)e determined by retention time and m/z value. Employing standard operating procedures for both sample preparation and ESI-mass spectrometry, one typically obtains under 1.34 min relention time precision, 0.3 amu mass precision and a median of RSD less than 22%, as well as a good linearity of the response curve for peptide ions over 3 orders of magnitude in concentration. Highly selective identification of discriminatory. peptides within a matrix of constitutively represented peptides using targeted MS/MS is achieved. The application of this method may represent a promising approach for quantitative peptidomics and discovery of peptide biomarkers.
关键词[WOS]: SERUM PROTEOMIC PATTERNS ;  CANCER-DIAGNOSIS ;  SPECTROMETRY ;  PEPTIDOMICS ;  MIXTURES ;  PROTEIN ;  MALDI
语种: 英语
WOS记录号: WOS:000258939700001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141010
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Li Xin,Xu Song-Yun,Zhang Yu,et al. Retention time mass-charge ratio pairs for label-free differential analysis of peptides[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(7):867-873.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Li Xin]'s Articles
 [Xu Song-Yun]'s Articles
 [Zhang Yu]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Li Xin]‘s Articles
 [Xu Song-Yun]‘s Articles
 [Zhang Yu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace