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Retention time mass-charge ratio pairs for label-free differential analysis of peptides
Li Xin; Xu Song-Yun; Zhang Yu; Zou Han-Fa
KeywordLabel-free Liquid Chromatography-mass Spectrometry Quantitative Proteomics Peptidome
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2008-07-01
Volume36Issue:7Pages:867-873
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSERUM PROTEOMIC PATTERNS ; CANCER-DIAGNOSIS ; SPECTROMETRY ; PEPTIDOMICS ; MIXTURES ; PROTEIN ; MALDI
AbstractA simple LC-MS based methodology is presented that allows relative changes in abundance of peptides In highly complex mixtures to he determined. Proposed method involves signal extraction, generation of features, alignment of corresponding features across different replicates/samples, and monitoring fold changes in relative abundance among different conditions without requiring the use of isotopic or metabolic labeling strategies. Utilizing a nano-flow chromatographic separation system along with the high mass resolution and mass accuracy of all electrospray-ionization (ESI) ion-trap mass spectrometer, the quantitative comparison of thousands of ions emanating front identically prepared control and experimental samples can be made. Using this configuration, the change in relative abundance of a small number of ions between the two conditions solely can I)e determined by retention time and m/z value. Employing standard operating procedures for both sample preparation and ESI-mass spectrometry, one typically obtains under 1.34 min relention time precision, 0.3 amu mass precision and a median of RSD less than 22%, as well as a good linearity of the response curve for peptide ions over 3 orders of magnitude in concentration. Highly selective identification of discriminatory. peptides within a matrix of constitutively represented peptides using targeted MS/MS is achieved. The application of this method may represent a promising approach for quantitative peptidomics and discovery of peptide biomarkers.
Language英语
WOS IDWOS:000258939700001
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141010
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li Xin,Xu Song-Yun,Zhang Yu,et al. Retention time mass-charge ratio pairs for label-free differential analysis of peptides[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(7):867-873.
APA Li Xin,Xu Song-Yun,Zhang Yu,&Zou Han-Fa.(2008).Retention time mass-charge ratio pairs for label-free differential analysis of peptides.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(7),867-873.
MLA Li Xin,et al."Retention time mass-charge ratio pairs for label-free differential analysis of peptides".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.7(2008):867-873.
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