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Retention time mass-charge ratio pairs for label-free differential analysis of peptides
Li Xin; Xu Song-Yun; Zhang Yu; Zou Han-Fa
关键词Label-free Liquid Chromatography-mass Spectrometry Quantitative Proteomics Peptidome
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2008-07-01
36期:7页:867-873
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SERUM PROTEOMIC PATTERNS ; CANCER-DIAGNOSIS ; SPECTROMETRY ; PEPTIDOMICS ; MIXTURES ; PROTEIN ; MALDI
英文摘要A simple LC-MS based methodology is presented that allows relative changes in abundance of peptides In highly complex mixtures to he determined. Proposed method involves signal extraction, generation of features, alignment of corresponding features across different replicates/samples, and monitoring fold changes in relative abundance among different conditions without requiring the use of isotopic or metabolic labeling strategies. Utilizing a nano-flow chromatographic separation system along with the high mass resolution and mass accuracy of all electrospray-ionization (ESI) ion-trap mass spectrometer, the quantitative comparison of thousands of ions emanating front identically prepared control and experimental samples can be made. Using this configuration, the change in relative abundance of a small number of ions between the two conditions solely can I)e determined by retention time and m/z value. Employing standard operating procedures for both sample preparation and ESI-mass spectrometry, one typically obtains under 1.34 min relention time precision, 0.3 amu mass precision and a median of RSD less than 22%, as well as a good linearity of the response curve for peptide ions over 3 orders of magnitude in concentration. Highly selective identification of discriminatory. peptides within a matrix of constitutively represented peptides using targeted MS/MS is achieved. The application of this method may represent a promising approach for quantitative peptidomics and discovery of peptide biomarkers.
语种英语
WOS记录号WOS:000258939700001
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141010
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Li Xin,Xu Song-Yun,Zhang Yu,et al. Retention time mass-charge ratio pairs for label-free differential analysis of peptides[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(7):867-873.
APA Li Xin,Xu Song-Yun,Zhang Yu,&Zou Han-Fa.(2008).Retention time mass-charge ratio pairs for label-free differential analysis of peptides.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(7),867-873.
MLA Li Xin,et al."Retention time mass-charge ratio pairs for label-free differential analysis of peptides".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.7(2008):867-873.
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