DICP OpenIR
Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method
Gai Qing-Qing1,3; Liu Qiu-Ye1; Li Wen-You1; He Xi-Wen1; Chen Lang-Xing1; Zhang Yu-Kui1,2
KeywordMolecular Imprinting Molecular Recognition Bovine Hemoglobin Photografting Iniferter
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2008-01-10
Volume29Issue:1Pages:64-70
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordSOLID-PHASE EXTRACTION ; LIQUID-CHROMATOGRAPHY ; RECOGNITION ; PROTEIN ; ALBUMIN ; SENSOR ; ACID
AbstractMolecularly imprinted polymers(MIP) based on photografting surface-modified polystyrene beads as matrices were prepared with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and N, N'-methylene bisacrylamide as the crosslinker in a phosphate buffer. The results of IR, scanning electron microscope(SEM) and elemental analysis demonstrate the formation of a grafting polymer layer on the Polystyrene-bead surface. Subsequent removal of the template leaves behind cavities on the surface of polymer matrix with a shape and an arrangement of functional groups in complementary binding sites to the original template. And the adsorption studies show that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. Our results demonstrated that the polymer prepared via photografting surface-modified method exhibited a better selectivity for the template. Attempt to employ the new method in molecular imprinting techniques may indroduce new application to MIPs and facillcate the probable protein separation and purification.
Language英语
WOS IDWOS:000252618000013
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141032
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
3.Changzhi Med Coll, Dept Pharmacol, Changzhi 046000, Peoples R China
Recommended Citation
GB/T 7714
Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,et al. Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2008,29(1):64-70.
APA Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,He Xi-Wen,Chen Lang-Xing,&Zhang Yu-Kui.(2008).Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,29(1),64-70.
MLA Gai Qing-Qing,et al."Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 29.1(2008):64-70.
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