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Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method
Gai Qing-Qing1,3; Liu Qiu-Ye1; Li Wen-You1; He Xi-Wen1; Chen Lang-Xing1; Zhang Yu-Kui1,2
关键词Molecular Imprinting Molecular Recognition Bovine Hemoglobin Photografting Iniferter
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2008-01-10
29期:1页:64-70
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]SOLID-PHASE EXTRACTION ; LIQUID-CHROMATOGRAPHY ; RECOGNITION ; PROTEIN ; ALBUMIN ; SENSOR ; ACID
英文摘要Molecularly imprinted polymers(MIP) based on photografting surface-modified polystyrene beads as matrices were prepared with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and N, N'-methylene bisacrylamide as the crosslinker in a phosphate buffer. The results of IR, scanning electron microscope(SEM) and elemental analysis demonstrate the formation of a grafting polymer layer on the Polystyrene-bead surface. Subsequent removal of the template leaves behind cavities on the surface of polymer matrix with a shape and an arrangement of functional groups in complementary binding sites to the original template. And the adsorption studies show that the imprinted polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. Our results demonstrated that the polymer prepared via photografting surface-modified method exhibited a better selectivity for the template. Attempt to employ the new method in molecular imprinting techniques may indroduce new application to MIPs and facillcate the probable protein separation and purification.
语种英语
WOS记录号WOS:000252618000013
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141032
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
3.Changzhi Med Coll, Dept Pharmacol, Changzhi 046000, Peoples R China
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GB/T 7714
Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,et al. Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2008,29(1):64-70.
APA Gai Qing-Qing,Liu Qiu-Ye,Li Wen-You,He Xi-Wen,Chen Lang-Xing,&Zhang Yu-Kui.(2008).Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,29(1),64-70.
MLA Gai Qing-Qing,et al."Preparation of bovine hemoglobin-imprinted polymer beads via photografting surface-modified method".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 29.1(2008):64-70.
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