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Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples
Wang, Yuan1; Lehmann, Rainer2; Lu, Xin1; Zhao, Xinjie1; Xu, Guowang1
KeywordHplc Column-switching Hilic Hydrophobic Hydrophilic Metabonomics
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2008-09-12
DOI10.1016/j.chroma.2008.07.010
Volume1204Issue:1Pages:28-34
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; COMPREHENSIVE 2-DIMENSIONAL SEPARATIONS ; REVERSED-PHASE ; FLUORESCENCE DETECTION ; MICRODIALYSIS SAMPLES ; PEAK-CAPACITY ; HUMAN PLASMA ; MS METHOD ; ESI-MS ; HPLC
Abstractin this study we developed and optimized a column-switching high-performance liquid chromatographic (HPLC) system for the complementary analysis of polar and apolar compounds in complex samples. The polar compounds are separated on a hydrophilic interaction chromatographic (HILIC) column; the part of the sample non-retained on HILIC is transferred into and separated on a reversed-phase (RP) column through an interface, thus preventing the loss of analytes eluting at dead time in common single-column mode. The signals are in turn recorded in the same chromatogram either by a UV or by a mass spectrometric detector. Applying a mixture of 25 standard compounds (12 hydrophilic and 13 hydrophobic) the system proves to be reliable, robust and easy-operating. The investigation of urine revealed an increase in the detected metabolite ion masses compared to the single-column HILIC-electrospray ionization (ESI)-MS analysis. Utilizing online the complementary selectivity of HILIC and RPLC in a fully automatic mode, this approach holds out the prospect to enlarge the number of detectable compounds in non-targeted "-omics" studies and sophisticated target-driven approaches by a single injection. (c) 2008 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000259516400004
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141071
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Hosp Tuebingen, Dept Internal Med 4, Cent Lab, Div Clin Chem & Pathobiochem, D-72076 Tubingen, Germany
Recommended Citation
GB/T 7714
Wang, Yuan,Lehmann, Rainer,Lu, Xin,et al. Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1204(1):28-34.
APA Wang, Yuan,Lehmann, Rainer,Lu, Xin,Zhao, Xinjie,&Xu, Guowang.(2008).Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples.JOURNAL OF CHROMATOGRAPHY A,1204(1),28-34.
MLA Wang, Yuan,et al."Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples".JOURNAL OF CHROMATOGRAPHY A 1204.1(2008):28-34.
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