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题名: Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis
作者: Xu, Weijia2;  Gao, Peng1;  Zhang, Xiaohui2;  Song, Liguo3
关键词: Ceftazidime ;  capillary electrophoresis ;  quality control
刊名: NEW MICROBIOLOGICA
发表日期: 2008-07-01
卷: 31, 期:3, 页:377-381
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: Good quality of antibiotic discs is a fundamental prerequisite to accurate antibiotic susceptibility tests. Capillary electrophoresis (CE) is a widely used method for quantitative analysis. Here, using ceftazidime as an example, we report an easy-to-perform strategy to determine ceftazidime content in discs. First, a serial of ceftazidime standard solutions was prepared to determine the detection linearity. Utilizing the background buffer containing 50 mmol/L Na2HPO4, 0.045 mmol/L P-cyclodextrin and 3.15 mmol/L Tris (hydroxymethyl) aminomethane, ceftazidime of concentrations ranging from 1.875 to 60 mu g/ml showed a linear response to detected peak areas. Subsequently, four kinds of ceftazidime discs were selected from different manufacturers. The discs were homogenized using 1 ml deionized water, and then detected by CE after filtration. The results showed that one kind of discs were of poor quality as further confirmed by disc diffusion tests using standard strains. This study proved the potential of CE as an easy choice to perform disc quality control under appropriate conditions.
关键词[WOS]: STAPHYLOCOCCUS-AUREUS ;  QUALITY-CONTROL ;  RESISTANCE ;  SUSCEPTIBILITY
语种: 英语
WOS记录号: WOS:000259559800011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141076
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Municipal Cent Hosp, Dalian 116021, Peoples R China
3.Dalian Econ & Technol Dev Zone, Ctr Dis Control, Dalian 116000, Peoples R China

Recommended Citation:
Xu, Weijia,Gao, Peng,Zhang, Xiaohui,et al. Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis[J]. NEW MICROBIOLOGICA,2008,31(3):377-381.
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