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Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis
Xu, Weijia2; Gao, Peng1; Zhang, Xiaohui2; Song, Liguo3
关键词Ceftazidime Capillary Electrophoresis Quality Control
刊名NEW MICROBIOLOGICA
2008-07-01
31期:3页:377-381
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]STAPHYLOCOCCUS-AUREUS ; QUALITY-CONTROL ; RESISTANCE ; SUSCEPTIBILITY
英文摘要Good quality of antibiotic discs is a fundamental prerequisite to accurate antibiotic susceptibility tests. Capillary electrophoresis (CE) is a widely used method for quantitative analysis. Here, using ceftazidime as an example, we report an easy-to-perform strategy to determine ceftazidime content in discs. First, a serial of ceftazidime standard solutions was prepared to determine the detection linearity. Utilizing the background buffer containing 50 mmol/L Na2HPO4, 0.045 mmol/L P-cyclodextrin and 3.15 mmol/L Tris (hydroxymethyl) aminomethane, ceftazidime of concentrations ranging from 1.875 to 60 mu g/ml showed a linear response to detected peak areas. Subsequently, four kinds of ceftazidime discs were selected from different manufacturers. The discs were homogenized using 1 ml deionized water, and then detected by CE after filtration. The results showed that one kind of discs were of poor quality as further confirmed by disc diffusion tests using standard strains. This study proved the potential of CE as an easy choice to perform disc quality control under appropriate conditions.
语种英语
WOS记录号WOS:000259559800011
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141076
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Dalian Municipal Cent Hosp, Dalian 116021, Peoples R China
3.Dalian Econ & Technol Dev Zone, Ctr Dis Control, Dalian 116000, Peoples R China
推荐引用方式
GB/T 7714
Xu, Weijia,Gao, Peng,Zhang, Xiaohui,et al. Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis[J]. NEW MICROBIOLOGICA,2008,31(3):377-381.
APA Xu, Weijia,Gao, Peng,Zhang, Xiaohui,&Song, Liguo.(2008).Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis.NEW MICROBIOLOGICA,31(3),377-381.
MLA Xu, Weijia,et al."Evaluation of Ceftazidime contents in antibiotic discs by capillary electrophoresis".NEW MICROBIOLOGICA 31.3(2008):377-381.
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