中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation
作者: Chen, Zhiyong1;  Xu, Li2;  Liang, Yuan1;  Wang, Jianbin1;  Zhao, Meiping1;  Li, Yuanzong1
关键词: HPLC ;  monolith ;  cryogel ;  nanoparticle ;  protein
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2008-02-22
DOI: 10.1016/j.chroma.2007.12.084
卷: 1182, 期:1, 页:128-131
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A supermacroporous monolithic cryogel was directly prepared by in situ cryo-copolymerization in a stainless steel cartridge (70 mm x 5.0 mm I.D.) using methacrylic acid (MAA) as functional monomer;and polyethylene glycol diacrylate (PEGDA) as crosslinker. The highly crosslinked (90%, molar ratio) poly(MAA-PEGDA) cryogel had more uniform supermacropores with a mean diameter of 25 p,m compared to the poly(acrylamide)-based cryogels. The viability of poly(MAA-PEGDA) cryogel as a medium was demonstrated for separations of lysozyme from chicken egg white (CEW) and water-soluble poly(N-isopropylacrylamide-co-3-(dimethylamino) propyl methacrylamide) (NIPAM-DMAPMA) nanoparticles from its crude reaction solution. The dynamic binding capacities of lysozyme and the polymeric nanoparticles were 4.51 x 10(-3) mu mol/ml and 33.4 mu g/ml, respectively. The lysozyme recovered from the above separations had a purity of more than 85%, and retained 90% of its enzymatic activity. (c) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: AFFINITY ;  PURIFICATION ;  COLUMNS
语种: 英语
WOS记录号: WOS:000253869800013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141079
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, BNLMS, Beijing 100871, Peoples R China
2.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet engn, Beijing 100871, Peoples R China

Recommended Citation:
Chen, Zhiyong,Xu, Li,Liang, Yuan,et al. Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation[J]. JOURNAL OF CHROMATOGRAPHY A,2008,1182(1):128-131.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Chen, Zhiyong]'s Articles
 [Xu, Li]'s Articles
 [Liang, Yuan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Chen, Zhiyong]‘s Articles
 [Xu, Li]‘s Articles
 [Liang, Yuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace