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题名: Direct electrochemistry of hemoglobin and myoglobin at didodecyldimethylammonium bromide-modified powder microelectrode and application for electrochemical detection of nitric oxide
作者: Guo, Zhimou1, 2;  Chen, Jian1, 2;  Liu, Huan2;  Cha, Chuansin2
关键词: hemoglobin ;  myoglobin ;  direct electrochemistry ;  powder microelectrode ;  nitric oxide
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2008-01-21
DOI: 10.1016/j.aca.2007.11.038
卷: 607, 期:1, 页:30-36
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Hemoglobin (Hb) and myoglobin (Mb) were immobilized at the didodecyldimethylammonium bromide (DDAB)-modified powder microelectrode (PME) to fabricate Hb-DDAB-PME and Mb-DDAB-PME. Direct electrochemistry of Hb and Mb were achieved on the DDAB-modified PME. The formal potential was -0.224V for Hb and -0.212V for Mb (vs. SCE). The apparent surface concentration of Hb and Mb at the electrode surface was 2.83 x 10(-8) and 9.94 x 10(-8) Mol cm(-2). The Hb-DDAB-PME and Mb-DDAB-PME were successfully applied for measurement of NO in vitro. The anodic current peaks for NO oxidation at +0.7 V and the cathodic current peaks for NO reduction at -0.85V on the CV curves were obtained on the modified electrodes. For detection of NO at +0.7 V, the sensitivity is 3.31 mA mu M-1 cm(-2) for Hb-DDAB-PME and 0.6 mA mu M-1 cm(-2) for Mb-DDAB-PME. The detection limit is 5 nM for Hb-DDAB-PME and 9 nM for Mb-DDAB-PME. The linear response range is 9-100 and 28-330 nM for Hb- and Mb-modified PME, respectively. For the electrochemical detection of NO at -0.85 V by using Hb-DDAB-PME, the detection sensitivity is 39.56 mu A mu M-1 cm(-2); the detection limit is as low as 0.2 mu M; and the linear response range is 1.90-28.08 mu M. (c) 2007 Elsevier B.V. All rights reserved.
关键词[WOS]: BY-LAYER FILMS ;  DIRECT ELECTRON-TRANSFER ;  SHELL NANOCLUSTER FILMS ;  HEME-PROTEINS ;  CLAY NANOPARTICLES ;  SURFACTANT FILMS ;  ASCORBIC-ACID ;  ELECTROCATALYSIS ;  BIOSENSOR ;  DNA
语种: 英语
WOS记录号: WOS:000252834200003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141083
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Wuhan Univ, Coll Chem & Mol Sci, Lab Electrochem, Wuhan 430072, Peoples R China

Recommended Citation:
Guo, Zhimou,Chen, Jian,Liu, Huan,et al. Direct electrochemistry of hemoglobin and myoglobin at didodecyldimethylammonium bromide-modified powder microelectrode and application for electrochemical detection of nitric oxide[J]. ANALYTICA CHIMICA ACTA,2008,607(1):30-36.
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