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题名: Determination of tetrodotoxin SD in rat plasma by reversed phase ion-pair high performance liquid chromatography
作者: Zheng Yong-Yi1;  Wang Yan1;  Zhang Ji1;  Wang Ren-Feng1;  Yan Chao1, 2
关键词: reversed phase ion pair high performance liquid chromatography ;  tetrodotoxin ;  plasma
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2008-05-01
卷: 36, 期:5, 页:588-592
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A reversed phase ion-pair HPLC method has been developed for the determination of tetrodotoxin ( M) in rat plasma. Water was added to plasma sample before vortex. Then precipitator precipitatant ( acetonitrile -methanol containing 0.5% acetic acid = 3: 1) was added, vortex once more. After centrifuging, the supernatant was injected into the HPLC system. The separation was performed using an Agilent Zorbax SB C(18) column (150 mm x 4.6 mm, 5 mu m), with 10% ACN-90% 8 mmol/L heptanesulfonic acid sodium and 0.005% trifluoroacetic acid (pH was adjusted to 5.0) as mobile phase. The detection wavelength was 196 nm, flow rate was 1.0 mL/min and the column was at room temperature. The detection limit of TTX standard was 0.1055 mg/L, the detection limit of TTX in rat plasma was 10.55 mg/L. The regression was linear over the TTX plasma concentration range of 21.1-211 mg/L, with r = 0.9989. The RSD of intra-day and inter-day was less than 3%. The method is rapid, accurate for the determination of TTX in plasma.
语种: 英语
WOS记录号: WOS:000257902600005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141086
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zheng Yong-Yi,Wang Yan,Zhang Ji,et al. Determination of tetrodotoxin SD in rat plasma by reversed phase ion-pair high performance liquid chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(5):588-592.
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