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题名: Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry
作者: Yang, Li1, 2;  Xiong, Aizhen1;  He, Yuqi1;  Wang, Zaiyong2;  Wang, Changhong1, 2;  Wang, Zhengtao1, 2;  Li, Wei3;  Yang, Ling3;  Hu, Zhibi1, 2
刊名: CHEMICAL RESEARCH IN TOXICOLOGY
发表日期: 2008-12-01
DOI: 10.1021/tx800225q
卷: 21, 期:12, 页:2280-2288
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Chemistry, Medicinal ;  Chemistry, Multidisciplinary ;  Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ;  Chemistry ;  Toxicology
英文摘要: Bile acids (BAs) are crucial for the diagnosis, follow-up, and prognostics of liver and intestinal disorders and other diseases affecting BA metabolism. A rapid, simple, and sensitive analytical method is needed to demonstrate the full metabolic profile and simultaneously determine the individual BAs in biological samples. In our present study, an ultraperformance liquid chromatography-mass spectrometry (UPLCMS) method has been established and validated for simultaneous quantitation of 22 BAs and a metabonomic study was performed based on the chemometric analysis of the serum samples from carbon tetrachloride (CCl(4))- and (alpha-naphthylisothiocyanate (ANIT)-induced liver failure rats. The optimal chromatographic condition was effected by UPLC (Acquity UPLC BEH column, 1.7 mu m, 2.1 mm x 100 min) using a linear gradient elution system of methanol-5 mM ammonium acetate containing 0.01% acetic acid after a simple-step deproteinization by precipitation. The separation of the 22 BAs can be finished in less than 12 min, and the concentrations of these BAs in rat serums were simultaneously determined using a selective ions monitoring mode. The method was validated with respect to repeatability (relative standard deviation < 9.78%) and accuracy (relative errors from -13.55 to 9.58%). The range of each BA was found from not detected (nd) to 8301 ng mL(-1), respectively. Furthermore, the developed method was successfully applied to the metabonomics analysis of BAs in CCl(4)- and ANIT-induced liver failure rats, using principle component analysis and canonical discriminant analysis. The serum samples from the two types of rat liver injury could be distinguished from each other and from the untreated animals according to the varieties of BAs. It indicated that the level of BAs could be considered as a sensitive parameter of hepatotoxicity induced by different chemical toxins. This novel metabonomics study of BAs based on the UPLC-MS profile provides not only an accurate quantitative assay of the serum concentrations of biomarkers but also a promising methodology for evaluation of liver injury.
关键词[WOS]: INTRAHEPATIC CHOLESTASIS ;  CARBON-TETRACHLORIDE ;  GAS-CHROMATOGRAPHY ;  SERUM ;  RATS ;  EXCRETION ;  PREGNANCY ;  PROFILES ;  WOMEN
语种: 英语
WOS记录号: WOS:000261736700008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141160
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Univ Tradit Chinese Med, Minist Educ, Key Lab Standardizat Chinese Med, Shanghai 201203, Peoples R China
2.Shanghai R&D Ctr Standardizat Tradit Chinese Med, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Li,Xiong, Aizhen,He, Yuqi,et al. Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry[J]. CHEMICAL RESEARCH IN TOXICOLOGY,2008,21(12):2280-2288.
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