DICP OpenIR
Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry
Yang, Li1,2; Xiong, Aizhen1; He, Yuqi1; Wang, Zaiyong2; Wang, Changhong1,2; Wang, Zhengtao1,2; Li, Wei3; Yang, Ling3; Hu, Zhibi1,2
刊名CHEMICAL RESEARCH IN TOXICOLOGY
2008-12-01
DOI10.1021/tx800225q
21期:12页:2280-2288
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Chemistry ; Toxicology
关键词[WOS]INTRAHEPATIC CHOLESTASIS ; CARBON-TETRACHLORIDE ; GAS-CHROMATOGRAPHY ; SERUM ; RATS ; EXCRETION ; PREGNANCY ; PROFILES ; WOMEN
英文摘要Bile acids (BAs) are crucial for the diagnosis, follow-up, and prognostics of liver and intestinal disorders and other diseases affecting BA metabolism. A rapid, simple, and sensitive analytical method is needed to demonstrate the full metabolic profile and simultaneously determine the individual BAs in biological samples. In our present study, an ultraperformance liquid chromatography-mass spectrometry (UPLCMS) method has been established and validated for simultaneous quantitation of 22 BAs and a metabonomic study was performed based on the chemometric analysis of the serum samples from carbon tetrachloride (CCl(4))- and (alpha-naphthylisothiocyanate (ANIT)-induced liver failure rats. The optimal chromatographic condition was effected by UPLC (Acquity UPLC BEH column, 1.7 mu m, 2.1 mm x 100 min) using a linear gradient elution system of methanol-5 mM ammonium acetate containing 0.01% acetic acid after a simple-step deproteinization by precipitation. The separation of the 22 BAs can be finished in less than 12 min, and the concentrations of these BAs in rat serums were simultaneously determined using a selective ions monitoring mode. The method was validated with respect to repeatability (relative standard deviation < 9.78%) and accuracy (relative errors from -13.55 to 9.58%). The range of each BA was found from not detected (nd) to 8301 ng mL(-1), respectively. Furthermore, the developed method was successfully applied to the metabonomics analysis of BAs in CCl(4)- and ANIT-induced liver failure rats, using principle component analysis and canonical discriminant analysis. The serum samples from the two types of rat liver injury could be distinguished from each other and from the untreated animals according to the varieties of BAs. It indicated that the level of BAs could be considered as a sensitive parameter of hepatotoxicity induced by different chemical toxins. This novel metabonomics study of BAs based on the UPLC-MS profile provides not only an accurate quantitative assay of the serum concentrations of biomarkers but also a promising methodology for evaluation of liver injury.
语种英语
WOS记录号WOS:000261736700008
引用统计
被引频次:61[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141160
专题中国科学院大连化学物理研究所
作者单位1.Shanghai Univ Tradit Chinese Med, Minist Educ, Key Lab Standardizat Chinese Med, Shanghai 201203, Peoples R China
2.Shanghai R&D Ctr Standardizat Tradit Chinese Med, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Yang, Li,Xiong, Aizhen,He, Yuqi,et al. Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry[J]. CHEMICAL RESEARCH IN TOXICOLOGY,2008,21(12):2280-2288.
APA Yang, Li.,Xiong, Aizhen.,He, Yuqi.,Wang, Zaiyong.,Wang, Changhong.,...&Hu, Zhibi.(2008).Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry.CHEMICAL RESEARCH IN TOXICOLOGY,21(12),2280-2288.
MLA Yang, Li,et al."Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry".CHEMICAL RESEARCH IN TOXICOLOGY 21.12(2008):2280-2288.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Li]的文章
[Xiong, Aizhen]的文章
[He, Yuqi]的文章
百度学术
百度学术中相似的文章
[Yang, Li]的文章
[Xiong, Aizhen]的文章
[He, Yuqi]的文章
必应学术
必应学术中相似的文章
[Yang, Li]的文章
[Xiong, Aizhen]的文章
[He, Yuqi]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。