DICP OpenIR
Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry
Yang, Li1,2; Xiong, Aizhen1; He, Yuqi1; Wang, Zaiyong2; Wang, Changhong1,2; Wang, Zhengtao1,2; Li, Wei3; Yang, Ling3; Hu, Zhibi1,2
Source PublicationCHEMICAL RESEARCH IN TOXICOLOGY
2008-12-01
DOI10.1021/tx800225q
Volume21Issue:12Pages:2280-2288
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectChemistry, Medicinal ; Chemistry, Multidisciplinary ; Toxicology
WOS Research AreaPharmacology & Pharmacy ; Chemistry ; Toxicology
WOS KeywordINTRAHEPATIC CHOLESTASIS ; CARBON-TETRACHLORIDE ; GAS-CHROMATOGRAPHY ; SERUM ; RATS ; EXCRETION ; PREGNANCY ; PROFILES ; WOMEN
AbstractBile acids (BAs) are crucial for the diagnosis, follow-up, and prognostics of liver and intestinal disorders and other diseases affecting BA metabolism. A rapid, simple, and sensitive analytical method is needed to demonstrate the full metabolic profile and simultaneously determine the individual BAs in biological samples. In our present study, an ultraperformance liquid chromatography-mass spectrometry (UPLCMS) method has been established and validated for simultaneous quantitation of 22 BAs and a metabonomic study was performed based on the chemometric analysis of the serum samples from carbon tetrachloride (CCl(4))- and (alpha-naphthylisothiocyanate (ANIT)-induced liver failure rats. The optimal chromatographic condition was effected by UPLC (Acquity UPLC BEH column, 1.7 mu m, 2.1 mm x 100 min) using a linear gradient elution system of methanol-5 mM ammonium acetate containing 0.01% acetic acid after a simple-step deproteinization by precipitation. The separation of the 22 BAs can be finished in less than 12 min, and the concentrations of these BAs in rat serums were simultaneously determined using a selective ions monitoring mode. The method was validated with respect to repeatability (relative standard deviation < 9.78%) and accuracy (relative errors from -13.55 to 9.58%). The range of each BA was found from not detected (nd) to 8301 ng mL(-1), respectively. Furthermore, the developed method was successfully applied to the metabonomics analysis of BAs in CCl(4)- and ANIT-induced liver failure rats, using principle component analysis and canonical discriminant analysis. The serum samples from the two types of rat liver injury could be distinguished from each other and from the untreated animals according to the varieties of BAs. It indicated that the level of BAs could be considered as a sensitive parameter of hepatotoxicity induced by different chemical toxins. This novel metabonomics study of BAs based on the UPLC-MS profile provides not only an accurate quantitative assay of the serum concentrations of biomarkers but also a promising methodology for evaluation of liver injury.
Language英语
WOS IDWOS:000261736700008
Citation statistics
Cited Times:67[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141160
Collection中国科学院大连化学物理研究所
Affiliation1.Shanghai Univ Tradit Chinese Med, Minist Educ, Key Lab Standardizat Chinese Med, Shanghai 201203, Peoples R China
2.Shanghai R&D Ctr Standardizat Tradit Chinese Med, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yang, Li,Xiong, Aizhen,He, Yuqi,et al. Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry[J]. CHEMICAL RESEARCH IN TOXICOLOGY,2008,21(12):2280-2288.
APA Yang, Li.,Xiong, Aizhen.,He, Yuqi.,Wang, Zaiyong.,Wang, Changhong.,...&Hu, Zhibi.(2008).Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry.CHEMICAL RESEARCH IN TOXICOLOGY,21(12),2280-2288.
MLA Yang, Li,et al."Bile Acids Metabonomic Study on the CCl(4)- and (alpha-Naphthylisothiocyanate-Induced Animal Models: Quantitative Analysis of 22 Bile Acids by Ultraperformance Liquid Chromatography-Mass Spectrometry".CHEMICAL RESEARCH IN TOXICOLOGY 21.12(2008):2280-2288.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Li]'s Articles
[Xiong, Aizhen]'s Articles
[He, Yuqi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Li]'s Articles
[Xiong, Aizhen]'s Articles
[He, Yuqi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Li]'s Articles
[Xiong, Aizhen]'s Articles
[He, Yuqi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.