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A facile microdialysis interface for on-line desalting and identification of proteins by nano-electrospray ionization mass spectrometry
Sun, Liangliang1,2; Duan, Jicheng1,2; Tao, Dingyin1,2; Liang, Zhen1,2; Zhang, Weibing1; Zhang, Lihua1; Zhang, Yukui1
刊名RAPID COMMUNICATIONS IN MASS SPECTROMETRY
2008-08-01
DOI10.1002/rcm.3622
22期:15页:2391-2397
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Analytical ; Spectroscopy
研究领域[WOS]Chemistry ; Spectroscopy
关键词[WOS]COMPLEX BIOLOGICAL SAMPLES ; CAPILLARY ELECTROPHORESIS ; DUAL MICRODIALYSIS ; ESI-MS/MS ; DEVICE ; PROTEOMICS ; DIGESTION ; CLEANUP ; PHASE ; LC
英文摘要The adverse effect of salts, especially inorganic salts, on electrospray ionization mass spectrometry (ESI-MS) is one of the most serious obstacles that might limit its application. Among the numerous desalting approaches, the microdialysis technique is favorable for large molecules, such as proteins. In this work, employing a hollow fiber membrane of cellulose acetate (MWCO 3000 Da), a simple, facile and efficient microdialysis interface with the dead volume of less than 1 mu L was constructed for the on-line desalting and identification of proteins dissolved in high salt concentration buffer by nano-ESI-MS. Furthermore, with counterflow added, the desalting procedure was accelerated, and could be finished within 1 min. This system was successfully applied to the analysis of myoglobin dissolved in either high concentration ammonium acetate or sodium chloride buffer. The experimental results showed that, by using such a microdialysis interface, the salt concentration, even as high as 1 M, could be decreased by at least 2 orders of magnitude, while sample loss was less than 10%, demonstrating the potential of such an interface in broadening the application of nano-ESI-MS in the analysis of large molecules. Copyright (c) 2008 John Wiley & Sons, Ltd.
语种英语
WOS记录号WOS:000258188400012
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141163
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Sun, Liangliang,Duan, Jicheng,Tao, Dingyin,et al. A facile microdialysis interface for on-line desalting and identification of proteins by nano-electrospray ionization mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2008,22(15):2391-2397.
APA Sun, Liangliang.,Duan, Jicheng.,Tao, Dingyin.,Liang, Zhen.,Zhang, Weibing.,...&Zhang, Yukui.(2008).A facile microdialysis interface for on-line desalting and identification of proteins by nano-electrospray ionization mass spectrometry.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,22(15),2391-2397.
MLA Sun, Liangliang,et al."A facile microdialysis interface for on-line desalting and identification of proteins by nano-electrospray ionization mass spectrometry".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 22.15(2008):2391-2397.
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