中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: A facile microdialysis interface for on-line desalting and identification of proteins by nano-electrospray ionization mass spectrometry
作者: Sun, Liangliang1, 2;  Duan, Jicheng1, 2;  Tao, Dingyin1, 2;  Liang, Zhen1, 2;  Zhang, Weibing1;  Zhang, Lihua1;  Zhang, Yukui1
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2008-08-01
DOI: 10.1002/rcm.3622
卷: 22, 期:15, 页:2391-2397
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Analytical ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Spectroscopy
英文摘要: The adverse effect of salts, especially inorganic salts, on electrospray ionization mass spectrometry (ESI-MS) is one of the most serious obstacles that might limit its application. Among the numerous desalting approaches, the microdialysis technique is favorable for large molecules, such as proteins. In this work, employing a hollow fiber membrane of cellulose acetate (MWCO 3000 Da), a simple, facile and efficient microdialysis interface with the dead volume of less than 1 mu L was constructed for the on-line desalting and identification of proteins dissolved in high salt concentration buffer by nano-ESI-MS. Furthermore, with counterflow added, the desalting procedure was accelerated, and could be finished within 1 min. This system was successfully applied to the analysis of myoglobin dissolved in either high concentration ammonium acetate or sodium chloride buffer. The experimental results showed that, by using such a microdialysis interface, the salt concentration, even as high as 1 M, could be decreased by at least 2 orders of magnitude, while sample loss was less than 10%, demonstrating the potential of such an interface in broadening the application of nano-ESI-MS in the analysis of large molecules. Copyright (c) 2008 John Wiley & Sons, Ltd.
关键词[WOS]: COMPLEX BIOLOGICAL SAMPLES ;  CAPILLARY ELECTROPHORESIS ;  DUAL MICRODIALYSIS ;  ESI-MS/MS ;  DEVICE ;  PROTEOMICS ;  DIGESTION ;  CLEANUP ;  PHASE ;  LC
语种: 英语
WOS记录号: WOS:000258188400012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141163
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Sun, Liangliang,Duan, Jicheng,Tao, Dingyin,et al. A facile microdialysis interface for on-line desalting and identification of proteins by nano-electrospray ionization mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2008,22(15):2391-2397.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Sun, Liangliang]'s Articles
 [Duan, Jicheng]'s Articles
 [Tao, Dingyin]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Sun, Liangliang]‘s Articles
 [Duan, Jicheng]‘s Articles
 [Tao, Dingyin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace