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题名: Design of parallel microfluidic gradient-generating networks for studying cellular response to chemical stimulus
作者: Wang Li-Hui2;  Liu Da-Yu3;  Bo, Wang1;  Jie, Sun1;  Li Lian-Hong1
关键词: microfluidic chip ;  gradient concentration ;  laminar flow ;  cell
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2008-02-01
卷: 36, 期:2, 页:143-149
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A microfluidic chip featuring laminar flow-based parallel gradient-generating networks was designedand fabricated. The microchip contains 5 gradient generators and 30 cell chambers, which resulted concentration gradients of drugs were delivered to stimulate the on-chip cultured cells. This microfluidies exploits the advantage of lab-on-a-chlip technology by integrating the generation of drug concentration gradients and a series of cell operations including seeding, culture, stimulation and staining into a chip. The microfluidic network was patterned on a glass wafer, which was further bonded to a polydimethylsiloxane ( PDMS) film. A series of Weir structures were fabricated on the cell culture reservoir to facilitate cell positioning and seeding. Injection of cell and delivery of medium and reagents were controlled by a syringe pump. Steady parallel concentration gradients were generated by flowing two fluids in each network. Over time observation showed the microchip was suitable for seeding and culture of MCF-7 cell. The microchip described above was applied in studying the role of reduced glutathione (GSH) in MCF-7 cells' chemotherapy sensitivity. MCF-7 breast cancer cell was treated with concentration gradients of As2O3 and N-acetyl cysteine (NAC) for GSH modulation, followed by exposure to adriamycin. GSH levels were down-regulated upon As2O3 treatment and up-regulated upon NAC treatment. Suppression of intracellular GSH by treatment with As2O3 has been shown to increase adriamycin sensitivity, conversely, elevation of intracellular GSH by treatment with NAC leads to the increased drug resistance. The integrated microfluidic chip is able to perform multiparametric pharmacological profiling with easy operation, thus holds great potential for extrapolation to the cell based high-content drug screening.
关键词[WOS]: GENE-EXPRESSION ;  GLUTATHIONE ;  COMPLEX ;  CHIP ;  CHEMOTAXIS ;  METABOLISM ;  SYSTEMS ;  CANCER ;  CELLS
语种: 英语
WOS记录号: WOS:000254252200002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141183
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Dept Pathol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Improve Med Instruments Co Ltd, Guangzhou 510370, Peoples R China

Recommended Citation:
Wang Li-Hui,Liu Da-Yu,Bo, Wang,et al. Design of parallel microfluidic gradient-generating networks for studying cellular response to chemical stimulus[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(2):143-149.
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