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题名: Resurveying the Tris buffer solution: The specific interaction between tris(hydroxymethyl)aminomethane and lysozyme
作者: Quan, Li1;  Wei, Dengguo1;  Jiang, Xiaolu1;  Liu, Yang1;  Li, Zhiyu1;  Li, Na1;  Li, Kean1;  Liu, Feng1;  Lai, Luhua1
关键词: lysozyme ;  tris(hydroxymethyl)aminomethane ;  protein-buffer binding ;  quartz crystal microbalance biosensor ;  surface plasmon resonance ;  molecular docking
刊名: ANALYTICAL BIOCHEMISTRY
发表日期: 2008-07-15
DOI: 10.1016/j.ab.2008.04.006
卷: 378, 期:2, 页:144-150
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An unusual phenomenon, the specific interaction between tris(hydroxymethyl)aminomethane (Tris) and lysozyme (LZM), was demonstrated for the first time by rapid screen analysis of interactions using a quartz crystal microbalance (QCM) biosensor. This phenomenon was also observed in a surface plasmon resonance (SPR) system. Further study using high-performance affinity chromatography (HPAC) confirmed this specific interaction between LZM and immobilized Tris with an apparent dissociation constant (K-D) of 6.7 x 10(-5) M. Molecular docking was carried out to identify possible modes of binding between LZM and Tris linked to a binding arm. The estimated binding free energy was -6.34 kcal mol(-1), corresponding to a K-D of 2.3 x 10(-5) M, which correlated well with the experimental value. Based on the docking model, the three hydroxyl groups of Tris form intermolecular H bonds with Asp52, Glu35, and Ala107 in LZM. This study reinforces the importance of buffer selection in quantitative biochemical investigations. For a lysozyme ligand binding study, it is better to avoid using Tris when the ligands under study are weak binders. (C) 2008 Elsevier Inc. All rights reserved.
关键词[WOS]: QUARTZ-CRYSTAL MICROBALANCE ;  AFFINITY-CHROMATOGRAPHY ;  BINDING ;  RECOGNITION ;  BIOSENSOR ;  LIGANDS ;  PROTEIN ;  SURFACE
语种: 英语
WOS记录号: WOS:000256846800006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141205
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem, Beijing 100871, Peoples R China

Recommended Citation:
Quan, Li,Wei, Dengguo,Jiang, Xiaolu,et al. Resurveying the Tris buffer solution: The specific interaction between tris(hydroxymethyl)aminomethane and lysozyme[J]. ANALYTICAL BIOCHEMISTRY,2008,378(2):144-150.
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