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题名: Comprehensive two-dimensional liquid chromatography (NPLC x RPLC) with vacuum-evaporation interface
作者: Tian, Hongzhe1, 2;  Xu, Jing1;  Guan, Yafeng1
关键词: comprehensive two-dimensional liquid chromatography ;  NPLC x RPLC ;  radix salviae miltiorrhiza bage ;  traditional Chinese medicines ;  vacuum-evaporation interface
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2008-06-01
DOI: 10.1002/jssc.200700559
卷: 31, 期:10, 页:1677-1685
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A comprehensive two-dimensional liquid chromatographic system incorporating a vacuum-evaporation interface was developed. Normal-phase liquid chromatography with a CN microcolumn was used as the first dimension (1(st)-D), and reversed-phase liquid chromatography with a C-18 monolithic column was used as the second dimension (2(nd) -D). An electronically controlled dual-position, ten-port valve with two identical storage loops served as the interface and the analysis time in the 2(nd)-D was 1.5 min. The solvent in the loops of the interface was evaporated at 25 degrees C under vacuum conditions, leaving the analytes on the inner wall of the loops. The mobile phase of the 2(nd) -D dissolved the analytes in the loop and injected them onto the second column, allowing an on-line solvent exchange of the fractions from the 1(st)-D to the 2(nd)-D. The chromatographic resolution of analytes on the two dimensions was evaluated. Sample loss due to evaporation in the interface was investigated with standard samples having different boiling points. The usefulness of the comprehensive 2-DLC system was demonstrated in the analysis of a traditional Chinese medicine Radix salviae miltiorrhiza bage extract.
关键词[WOS]: SEPARATION
语种: 英语
WOS记录号: WOS:000256987500004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141235
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Shenyang Agr Univ, Analyt Ctr, Shenyang, Peoples R China

Recommended Citation:
Tian, Hongzhe,Xu, Jing,Guan, Yafeng. Comprehensive two-dimensional liquid chromatography (NPLC x RPLC) with vacuum-evaporation interface[J]. JOURNAL OF SEPARATION SCIENCE,2008,31(10):1677-1685.
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