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Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin
Liu Qiu-Ye1; He Xi-Wen1; Li Wen-You1; Chen Lang-Xing1; Zhang Yu-Kui1,2
关键词Molecular Imprinted Polymer Bovine Hemoglobin Silica Gel Chitosan
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2009-04-10
30期:4页:691-696
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]RECOGNITION ; SURFACE ; POLYMER ; GEL ; TEMPLATES
英文摘要The silica modified with chitosan was synthesized as a carrier material. The glutaraldehyde was coupled with amido group of chitosan, and template protein-bovine hemoglobin was immobilized by aldehyde group. Then, the carrier was coated by sol-gel, after template protein was removed; molecularly imprinted polymer was prepared for selectively recognizing bovine hemoglobin (BHb). The results of IR and scanning electron microscope(SEM) demonstrated the formation of a grafting polymer layer on the modified silica gel surface. Selectivity adsorption experiments under equilibrium adsorption show that the imprinted polymers have good adsorption capacity and specific recognition for BHb in aqueous solution, and imprinted polymer hold good recognition capability for BHb when completive substances are present. The MIP synthesis strategy is promising for separating and purifying specific protein compositions in bio-samples. It may have good prospect for riching target protein, which is required to further study.
语种英语
WOS记录号WOS:000265665700010
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141259
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Liu Qiu-Ye,He Xi-Wen,Li Wen-You,et al. Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(4):691-696.
APA Liu Qiu-Ye,He Xi-Wen,Li Wen-You,Chen Lang-Xing,&Zhang Yu-Kui.(2009).Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,30(4),691-696.
MLA Liu Qiu-Ye,et al."Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 30.4(2009):691-696.
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