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题名: Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin
作者: Liu Qiu-Ye1;  He Xi-Wen1;  Li Wen-You1;  Chen Lang-Xing1;  Zhang Yu-Kui1, 2
关键词: Molecular imprinted polymer ;  Bovine hemoglobin ;  Silica gel ;  Chitosan
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2009-04-10
卷: 30, 期:4, 页:691-696
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: The silica modified with chitosan was synthesized as a carrier material. The glutaraldehyde was coupled with amido group of chitosan, and template protein-bovine hemoglobin was immobilized by aldehyde group. Then, the carrier was coated by sol-gel, after template protein was removed; molecularly imprinted polymer was prepared for selectively recognizing bovine hemoglobin (BHb). The results of IR and scanning electron microscope(SEM) demonstrated the formation of a grafting polymer layer on the modified silica gel surface. Selectivity adsorption experiments under equilibrium adsorption show that the imprinted polymers have good adsorption capacity and specific recognition for BHb in aqueous solution, and imprinted polymer hold good recognition capability for BHb when completive substances are present. The MIP synthesis strategy is promising for separating and purifying specific protein compositions in bio-samples. It may have good prospect for riching target protein, which is required to further study.
关键词[WOS]: RECOGNITION ;  SURFACE ;  POLYMER ;  GEL ;  TEMPLATES
语种: 英语
WOS记录号: WOS:000265665700010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141259
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Liu Qiu-Ye,He Xi-Wen,Li Wen-You,et al. Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(4):691-696.
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