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题名: Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging
作者: Wu, Jing1;  Ye, Zhiqiang2;  Wang, Guilan2;  Jin, Dayong3;  Yuan, Jingli1, 2;  Guan, Yafeng1;  Piper, James3
刊名: JOURNAL OF MATERIALS CHEMISTRY
发表日期: 2009
DOI: 10.1039/b815999h
卷: 19, 期:9, 页:1258-1264
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Physical ;  Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: Time-gated luminescence bioimaging based on microsecond-lifetime luminescent biolabels can provide complete background-free conditions for detecting target cells in an autofluorescence biosample matrix. However, a major drawback of the current lanthanide biolabels is the requirement for UV excitation (<370 nm), which leads to damage to many biological systems and greatly affects the improvement of time-gated luminescence instruments. Herein we describe luminescent europium nanoparticles that have an excitation peak around 406 nm with high quantum yield (similar to 66%) and fine monodispersity in aqueous solutions. The nanoparticles were prepared by copolymerization of a visible-light-sensitized Eu(3+) complex 4,4'-bis(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl) chlorosulfo-o-terphenyl-Eu(3+)-2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine conjugated with 3-aminopropyl(triethoxy) silane, free 3-aminopropyl(triethoxy) silane and tetraethyl orthosilicate in a water-in-oil reverse microemulsion. Characterization by transmission electron microscopy and luminescence spectroscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, <50 nm in diameter, and show strong visible-light-sensitized luminescence with a large quantum yield and a long luminescence lifetime. The new nanoparticles were successfully applied to distinguish an environmental pathogen, Giardia lamblia, within a concentrate of environmental water sample using a time-gated luminescence microscope with pulsed visible light excitation. The method resulted in highly specific and sensitive imaging for Giardia lamblia. These results suggest a broad range of potential bioimaging applications where both long time microscopy observation and high signal-to-background ratio are required for samples containing high concentrations of autofluorescence background.
关键词[WOS]: RESOLVED FLUOROMETRIC APPLICATION ;  DOPED SILICA NANOPARTICLES ;  PROSTATE-SPECIFIC ANTIGEN ;  BIOCONJUGATED NANOPARTICLES ;  FLUORESCENT NANOPARTICLES ;  CRYPTOSPORIDIUM-PARVUM ;  QUANTUM DOTS ;  CHELATE ;  EXCITATION ;  MICROSCOPY
语种: 英语
WOS记录号: WOS:000263450300007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141273
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Macquarie Univ, Div Informat & Commun Sci, MQ Photon Ctr, N Ryde, NSW 2109, Australia

Recommended Citation:
Wu, Jing,Ye, Zhiqiang,Wang, Guilan,et al. Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging[J]. JOURNAL OF MATERIALS CHEMISTRY,2009,19(9):1258-1264.
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