DICP OpenIR
Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging
Wu, Jing1; Ye, Zhiqiang2; Wang, Guilan2; Jin, Dayong3; Yuan, Jingli1,2; Guan, Yafeng1; Piper, James3
Source PublicationJOURNAL OF MATERIALS CHEMISTRY
2009
DOI10.1039/b815999h
Volume19Issue:9Pages:1258-1264
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Physical ; Materials Science, Multidisciplinary
WOS Research AreaChemistry ; Materials Science
WOS KeywordRESOLVED FLUOROMETRIC APPLICATION ; DOPED SILICA NANOPARTICLES ; PROSTATE-SPECIFIC ANTIGEN ; BIOCONJUGATED NANOPARTICLES ; FLUORESCENT NANOPARTICLES ; CRYPTOSPORIDIUM-PARVUM ; QUANTUM DOTS ; CHELATE ; EXCITATION ; MICROSCOPY
AbstractTime-gated luminescence bioimaging based on microsecond-lifetime luminescent biolabels can provide complete background-free conditions for detecting target cells in an autofluorescence biosample matrix. However, a major drawback of the current lanthanide biolabels is the requirement for UV excitation (<370 nm), which leads to damage to many biological systems and greatly affects the improvement of time-gated luminescence instruments. Herein we describe luminescent europium nanoparticles that have an excitation peak around 406 nm with high quantum yield (similar to 66%) and fine monodispersity in aqueous solutions. The nanoparticles were prepared by copolymerization of a visible-light-sensitized Eu(3+) complex 4,4'-bis(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl) chlorosulfo-o-terphenyl-Eu(3+)-2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine conjugated with 3-aminopropyl(triethoxy) silane, free 3-aminopropyl(triethoxy) silane and tetraethyl orthosilicate in a water-in-oil reverse microemulsion. Characterization by transmission electron microscopy and luminescence spectroscopy indicates that the nanoparticles are monodisperse, spherical and uniform in size, <50 nm in diameter, and show strong visible-light-sensitized luminescence with a large quantum yield and a long luminescence lifetime. The new nanoparticles were successfully applied to distinguish an environmental pathogen, Giardia lamblia, within a concentrate of environmental water sample using a time-gated luminescence microscope with pulsed visible light excitation. The method resulted in highly specific and sensitive imaging for Giardia lamblia. These results suggest a broad range of potential bioimaging applications where both long time microscopy observation and high signal-to-background ratio are required for samples containing high concentrations of autofluorescence background.
Language英语
WOS IDWOS:000263450300007
Citation statistics
Cited Times:85[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141273
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Macquarie Univ, Div Informat & Commun Sci, MQ Photon Ctr, N Ryde, NSW 2109, Australia
Recommended Citation
GB/T 7714
Wu, Jing,Ye, Zhiqiang,Wang, Guilan,et al. Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging[J]. JOURNAL OF MATERIALS CHEMISTRY,2009,19(9):1258-1264.
APA Wu, Jing.,Ye, Zhiqiang.,Wang, Guilan.,Jin, Dayong.,Yuan, Jingli.,...&Piper, James.(2009).Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging.JOURNAL OF MATERIALS CHEMISTRY,19(9),1258-1264.
MLA Wu, Jing,et al."Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging".JOURNAL OF MATERIALS CHEMISTRY 19.9(2009):1258-1264.
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