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Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica
Zhou Xue1,2; He Xi-Wen1; Chen Lang-Xing1; Li Wen-You1; Zhang Yu-Kui1,3
KeywordSurface Imprinting Separation Selectivity Recognition Mechanism Bovine Serum Albumin
Source PublicationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2009-02-01
Volume37Issue:2Pages:174-180
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordRECOGNITION ; PEPTIDES ; PROTEINS ; SENSORS
AbstractSol-gel molecularly imprinted polymers for recognition of bovine serum albumin were prepared via polymerization on the surface of aminopropyl silica followed by immobilization proteins. Based on the above system, the relationship between fundamental parameters of preparation of molecularly imprinted polymers and their effect on the separation selectivity was studied. A mixture of octyltrimethoxysilane, 3-aminopropylsilane and tetraethoxysilane at ratio of 42.5:42.5:15 were polymerized at pH 7.0 before template protein removal by solution of 0.1 mol/L NaOH and 10% (V/V) HAc-10% (W/V) SDS. The results indicated that tire molecularly imprinted polymers obtained by this method showed the best separation selectivity. Moreover, the recognition mechanism of template proteins was analyzed preliminarily.
Language英语
WOS IDWOS:000265169000003
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141287
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Anshan Normal Coll, Dept Chem, Anshan 114007, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zhou Xue,He Xi-Wen,Chen Lang-Xing,et al. Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(2):174-180.
APA Zhou Xue,He Xi-Wen,Chen Lang-Xing,Li Wen-You,&Zhang Yu-Kui.(2009).Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(2),174-180.
MLA Zhou Xue,et al."Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.2(2009):174-180.
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