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题名: Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica
作者: Zhou Xue1, 2;  He Xi-Wen1;  Chen Lang-Xing1;  Li Wen-You1;  Zhang Yu-Kui1, 3
关键词: Surface imprinting ;  separation selectivity ;  recognition mechanism ;  bovine serum albumin
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2009-02-01
卷: 37, 期:2, 页:174-180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Sol-gel molecularly imprinted polymers for recognition of bovine serum albumin were prepared via polymerization on the surface of aminopropyl silica followed by immobilization proteins. Based on the above system, the relationship between fundamental parameters of preparation of molecularly imprinted polymers and their effect on the separation selectivity was studied. A mixture of octyltrimethoxysilane, 3-aminopropylsilane and tetraethoxysilane at ratio of 42.5:42.5:15 were polymerized at pH 7.0 before template protein removal by solution of 0.1 mol/L NaOH and 10% (V/V) HAc-10% (W/V) SDS. The results indicated that tire molecularly imprinted polymers obtained by this method showed the best separation selectivity. Moreover, the recognition mechanism of template proteins was analyzed preliminarily.
关键词[WOS]: RECOGNITION ;  PEPTIDES ;  PROTEINS ;  SENSORS
语种: 英语
WOS记录号: WOS:000265169000003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141287
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Anshan Normal Coll, Dept Chem, Anshan 114007, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Zhou Xue,He Xi-Wen,Chen Lang-Xing,et al. Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(2):174-180.
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