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Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA
Lv Wei1,3; Chen XuDong1,3; Aumiler, Damir1,2,3; Xia AnDong1,3
关键词Cyanine Dyes Single Molecule Spectroscopy On/off Blinking Fluorescence Correlation Spectroscopy (Fcs)
刊名SCIENCE IN CHINA SERIES B-CHEMISTRY
2009-08-01
DOI10.1007/s11426-009-0059-2
52期:8页:1148-1153
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]PHOTOINDUCED ISOMERIZATION ; BACK-ISOMERIZATION ; ROOM-TEMPERATURE ; GLASS-SURFACE ; CY5 ; SPECTROSCOPY ; INTENSITY ; PHOTOPHYSICS
英文摘要The intersystem crossing and isomerization dynamics of free-Cy3, Cy3-ssDNA, free-Cy5 and Cy5-ssDNA are obtained through simple analysis of rapid on/off blinking from single molecule fluorescence intensity time-traces and the fluorescence correlation spectroscopy (FCS). The on- and off-times observed in fluorescence time traces of single cyanine dyes are due to the formation of the triplet state and isomerization, where both the interaction with DNA and long central polymethine chain of cyanine dyes increase the barriers of isomerization, leading to long off-time. The results indicate that the single molecule fluorescence fluctuation together with the resulting second autocorrelation analysis are powerful methods for determining the triplet state and isomerization dynamics, which could be the simple techniques and complementary to other spectroscopic techniques, such as fluorescence decay measurement and laser flash photolysis to study the photophysical processes of complex molecules.
语种英语
WOS记录号WOS:000268514800012
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141291
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100190, Peoples R China
2.Inst Phys, Zagreb 10000, Croatia
3.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100190, Peoples R China
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GB/T 7714
Lv Wei,Chen XuDong,Aumiler, Damir,et al. Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(8):1148-1153.
APA Lv Wei,Chen XuDong,Aumiler, Damir,&Xia AnDong.(2009).Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(8),1148-1153.
MLA Lv Wei,et al."Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.8(2009):1148-1153.
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