DICP OpenIR
Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA
Lv Wei1,3; Chen XuDong1,3; Aumiler, Damir1,2,3; Xia AnDong1,3
KeywordCyanine Dyes Single Molecule Spectroscopy On/off Blinking Fluorescence Correlation Spectroscopy (Fcs)
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
2009-08-01
DOI10.1007/s11426-009-0059-2
Volume52Issue:8Pages:1148-1153
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordPHOTOINDUCED ISOMERIZATION ; BACK-ISOMERIZATION ; ROOM-TEMPERATURE ; GLASS-SURFACE ; CY5 ; SPECTROSCOPY ; INTENSITY ; PHOTOPHYSICS
AbstractThe intersystem crossing and isomerization dynamics of free-Cy3, Cy3-ssDNA, free-Cy5 and Cy5-ssDNA are obtained through simple analysis of rapid on/off blinking from single molecule fluorescence intensity time-traces and the fluorescence correlation spectroscopy (FCS). The on- and off-times observed in fluorescence time traces of single cyanine dyes are due to the formation of the triplet state and isomerization, where both the interaction with DNA and long central polymethine chain of cyanine dyes increase the barriers of isomerization, leading to long off-time. The results indicate that the single molecule fluorescence fluctuation together with the resulting second autocorrelation analysis are powerful methods for determining the triplet state and isomerization dynamics, which could be the simple techniques and complementary to other spectroscopic techniques, such as fluorescence decay measurement and laser flash photolysis to study the photophysical processes of complex molecules.
Language英语
WOS IDWOS:000268514800012
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141291
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100190, Peoples R China
2.Inst Phys, Zagreb 10000, Croatia
3.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Lv Wei,Chen XuDong,Aumiler, Damir,et al. Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(8):1148-1153.
APA Lv Wei,Chen XuDong,Aumiler, Damir,&Xia AnDong.(2009).Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(8),1148-1153.
MLA Lv Wei,et al."Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.8(2009):1148-1153.
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