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题名: Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA
作者: Lv Wei1, 3;  Chen XuDong1, 3;  Aumiler, Damir1, 2, 3;  Xia AnDong1, 3
关键词: cyanine dyes ;  single molecule spectroscopy ;  on/off blinking ;  fluorescence correlation spectroscopy (FCS)
刊名: SCIENCE IN CHINA SERIES B-CHEMISTRY
发表日期: 2009-08-01
DOI: 10.1007/s11426-009-0059-2
卷: 52, 期:8, 页:1148-1153
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: The intersystem crossing and isomerization dynamics of free-Cy3, Cy3-ssDNA, free-Cy5 and Cy5-ssDNA are obtained through simple analysis of rapid on/off blinking from single molecule fluorescence intensity time-traces and the fluorescence correlation spectroscopy (FCS). The on- and off-times observed in fluorescence time traces of single cyanine dyes are due to the formation of the triplet state and isomerization, where both the interaction with DNA and long central polymethine chain of cyanine dyes increase the barriers of isomerization, leading to long off-time. The results indicate that the single molecule fluorescence fluctuation together with the resulting second autocorrelation analysis are powerful methods for determining the triplet state and isomerization dynamics, which could be the simple techniques and complementary to other spectroscopic techniques, such as fluorescence decay measurement and laser flash photolysis to study the photophysical processes of complex molecules.
关键词[WOS]: PHOTOINDUCED ISOMERIZATION ;  BACK-ISOMERIZATION ;  ROOM-TEMPERATURE ;  GLASS-SURFACE ;  CY5 ;  SPECTROSCOPY ;  INTENSITY ;  PHOTOPHYSICS
语种: 英语
WOS记录号: WOS:000268514800012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141291
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, Beijing 100190, Peoples R China
2.Inst Phys, Zagreb 10000, Croatia
3.Chinese Acad Sci, Inst Chem, BNLMS, Beijing 100190, Peoples R China

Recommended Citation:
Lv Wei,Chen XuDong,Aumiler, Damir,et al. Single molecule fluorescence fluctuations of the cyanine dyes linked covalently to DNA[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(8):1148-1153.
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