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题名: Multiple sequence elements determine the intron retention in histamine H-3 receptors in rats and mice
作者: Ding, Wenyong1, 2, 3;  Lin, Lin2;  Xiao, Zhifeng1;  Zou, Hanfa3;  Duan, Ziyuan1;  Dai, Jianwu1
关键词: Histamine H-3 receptor ;  Alternative splicing ;  Intron retention ;  Splice site ;  Sequence element
刊名: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
发表日期: 2009-11-01
DOI: 10.1016/j.biocel.2009.05.002
卷: 41, 期:11, 页:2281-2286
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Cell Biology
英文摘要: Intron retention in histamine H-3 receptors has been found in many mammals, including rats and mice The short transcript isoforms that exclude alternatively spliced introns are readily detected in very low abundance in rats and are undetectable in mice using the regular PCR approach. The detailed mechanism for the special alternative splicing remains poorly understood. The aim of this work was to investigate the effects of essential splice signals on intron retention and to identify sequence elements that determine the differences in splicing between rats and mice We have constructed a minigene-splicing system to mimic natural alternative receptor splicing and analyzed the regulatory elements in HEK293 cells. Mutating sub-optimal 5' and 3' splice sites toward the consensus sequences can enhance the splicing of corresponding alternative introns. The effect is much more significant for the 3' splice site of the longer intron than for the other two splice sites; it is also more significant in rats than in mice. The splicing differences between rats and mice are primarily determined by the six discrepant nucleotides within the alternative introns, which promote or suppress the intron splicing in different ways and cooperate to make splicing of the two introns more efficient in rats From these results we conclude that (1) the weakness of the splice sites is an important determinant for very low efficiency during intron splicing and, (2) multiple sequence elements determine the splicing differences between rats and mice. The results provide insight into special alternative splicing regulation in H-3 receptors. (C) 2009 Elsevier Ltd. All rights reserved
关键词[WOS]: OVERLAP EXTENSION ;  SPLICE SITES ;  ISOFORMS ;  CLONING ;  GENE ;  EXPRESSION ;  ORGANIZATION ;  PCR ;  RECOGNITION ;  MICROARRAYS
语种: 英语
WOS记录号: WOS:000271124700024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141297
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China
2.Dalian Med Univ, Dept Biochem & Mol Biol, Dalian 116044, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Ding, Wenyong,Lin, Lin,Xiao, Zhifeng,et al. Multiple sequence elements determine the intron retention in histamine H-3 receptors in rats and mice[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2009,41(11):2281-2286.
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