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Dynamic Insight into the Interaction between Porphyrin and G-quadruplex DNAs: Time-Resolved Fluorescence Anisotropy Study
Jia, Guoqing1,2; Feng, Zhaochi1; Wei, Chunying1; Zhou, Jun1,2; Wang, Xiuli1,2; Li, Can1
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY B
2009-12-17
DOI10.1021/jp906060d
Volume113Issue:50Pages:16237-16245
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Physical
WOS Research AreaChemistry
WOS KeywordANTICANCER DRUG DESIGN ; TELOMERIC DNA ; CATIONIC PORPHYRINS ; CIRCULAR-DICHROISM ; STRUCTURAL BASIS ; SMALL-MOLECULE ; NUCLEIC-ACIDS ; BINDING ; DEPOLARIZATION ; PROMOTER
AbstractUnderstanding the nature of the interaction between small molecules and G-quadruplex DNA is crucial for the development of novel anticancer drugs. In this paper, we present the first data on time-resolved fluorescence anisotropy Study on the interaction between a water-soluble cationic porphyrin H(2)TMPyP4 and four distinct G-quadruplex DNAs, that is, AG(3)(TAG(3))(3), thrombin-binding aptamer (TBA), (G(4)T(4)G(4))(2), and (TG(4)T)4. The anisotropy decay Curves show the monoexponential for free H(2)TMPyP4 and the biexponential upon binding to the excess amount of G-quadruplex DNAs. The biexponential anisotropy decay can be well interpreted using a wobbling-in-the-cone model. The orientational diffusion of the bound H(2)TMPyP4 is initially restricted to a limited cone angle within the G-quadruplex DNAs, and then an overall orientational relaxation of the G-quadruplex DNA-H(2)TMPyP4 complexes occurs in a longer time scale. It was found that the dynamics of the restricted internal rotation of bound H(2)TMPyP4 strongly depends on the ending structures of the G-quadruplex DNAs. According to the order parameter (Q) calculated front the wobbling-in-the-cone model, we deduce that the degree of restriction around the bound H(2)TMPyP4 follows the order of TBA > (TG(4)T)4 > AG(3)(T(2)AG(3))(3) > (G(4)T(4)G(4))(2). Especially, based on the maximum order parameter (Q) of bound H(2)TMPyP4 within TBA, a new sandwich-type binding mode for TBA-H(2)TMPyP4 complex was proposed in which both terminal G-quartet and T center dot T base pair stack on the porphyrin ring through pi-pi interaction. This study thus provides a new insight into the interaction between G-quadruplex DNAs and H(2)TMPyP4.
Language英语
WOS IDWOS:000272560100017
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141298
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Jia, Guoqing,Feng, Zhaochi,Wei, Chunying,et al. Dynamic Insight into the Interaction between Porphyrin and G-quadruplex DNAs: Time-Resolved Fluorescence Anisotropy Study[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(50):16237-16245.
APA Jia, Guoqing,Feng, Zhaochi,Wei, Chunying,Zhou, Jun,Wang, Xiuli,&Li, Can.(2009).Dynamic Insight into the Interaction between Porphyrin and G-quadruplex DNAs: Time-Resolved Fluorescence Anisotropy Study.JOURNAL OF PHYSICAL CHEMISTRY B,113(50),16237-16245.
MLA Jia, Guoqing,et al."Dynamic Insight into the Interaction between Porphyrin and G-quadruplex DNAs: Time-Resolved Fluorescence Anisotropy Study".JOURNAL OF PHYSICAL CHEMISTRY B 113.50(2009):16237-16245.
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