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题名: Dynamic Insight into the Interaction between Porphyrin and G-quadruplex DNAs: Time-Resolved Fluorescence Anisotropy Study
作者: Jia, Guoqing1, 2;  Feng, Zhaochi1;  Wei, Chunying1;  Zhou, Jun1, 2;  Wang, Xiuli1, 2;  Li, Can1
刊名: JOURNAL OF PHYSICAL CHEMISTRY B
发表日期: 2009-12-17
DOI: 10.1021/jp906060d
卷: 113, 期:50, 页:16237-16245
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Physical
研究领域[WOS]: Chemistry
英文摘要: Understanding the nature of the interaction between small molecules and G-quadruplex DNA is crucial for the development of novel anticancer drugs. In this paper, we present the first data on time-resolved fluorescence anisotropy Study on the interaction between a water-soluble cationic porphyrin H(2)TMPyP4 and four distinct G-quadruplex DNAs, that is, AG(3)(TAG(3))(3), thrombin-binding aptamer (TBA), (G(4)T(4)G(4))(2), and (TG(4)T)4. The anisotropy decay Curves show the monoexponential for free H(2)TMPyP4 and the biexponential upon binding to the excess amount of G-quadruplex DNAs. The biexponential anisotropy decay can be well interpreted using a wobbling-in-the-cone model. The orientational diffusion of the bound H(2)TMPyP4 is initially restricted to a limited cone angle within the G-quadruplex DNAs, and then an overall orientational relaxation of the G-quadruplex DNA-H(2)TMPyP4 complexes occurs in a longer time scale. It was found that the dynamics of the restricted internal rotation of bound H(2)TMPyP4 strongly depends on the ending structures of the G-quadruplex DNAs. According to the order parameter (Q) calculated front the wobbling-in-the-cone model, we deduce that the degree of restriction around the bound H(2)TMPyP4 follows the order of TBA > (TG(4)T)4 > AG(3)(T(2)AG(3))(3) > (G(4)T(4)G(4))(2). Especially, based on the maximum order parameter (Q) of bound H(2)TMPyP4 within TBA, a new sandwich-type binding mode for TBA-H(2)TMPyP4 complex was proposed in which both terminal G-quartet and T center dot T base pair stack on the porphyrin ring through pi-pi interaction. This study thus provides a new insight into the interaction between G-quadruplex DNAs and H(2)TMPyP4.
关键词[WOS]: ANTICANCER DRUG DESIGN ;  TELOMERIC DNA ;  CATIONIC PORPHYRINS ;  CIRCULAR-DICHROISM ;  STRUCTURAL BASIS ;  SMALL-MOLECULE ;  NUCLEIC-ACIDS ;  BINDING ;  DEPOLARIZATION ;  PROMOTER
语种: 英语
WOS记录号: WOS:000272560100017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141298
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Jia, Guoqing,Feng, Zhaochi,Wei, Chunying,et al. Dynamic Insight into the Interaction between Porphyrin and G-quadruplex DNAs: Time-Resolved Fluorescence Anisotropy Study[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2009,113(50):16237-16245.
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