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题名: Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution
作者: Zhang, Wei1;  Qin, Lei1;  He, Xi-Wen1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
关键词: Surface molecular imprinting ;  Silica beads ;  beta-Cyclodextrin ;  Lysozyme ;  High-performance liquid chromatography ;  Protein separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-05-22
DOI: 10.1016/j.chroma.2009.03.056
卷: 1216, 期:21, 页:4560-4567
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel protein imprinted polymer for selective recognition of lysozyme was obtained. Acryloy-beta-cyclodextrin, which offered hydrophilic exterior and hydrophobic cavity that were allowed to self-assemble with the template protein through hydrogen interaction and hydrophobic interaction, was synthesized and used as the functional monomer. Polymerization was carried out in the presence of acrylamide as an assistant monomer, which resulted in a new type of protein imprinted polymer. Langmuir adsorption model was employed to describe the isotherms. and maximum adsorption capacity was evaluated. The performance of such imprinted polymer was further demonstrated by high-performance liquid chromatography, and the results showed that the column packed with the lysozyme imprinted silica beads could effectively separate lysozyme from the mixture of lysozyme-cytochrome c, lysozyme-bovine serum albumin, lysozyme-avidin or lysozyme-methylated bovine serum albumin, which showed its high selectivity. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: PROTEIN RECOGNITION ;  ANTIBODIES ;  TRYPTOPHAN ;  SEPARATION ;  PEPTIDES ;  MEDIA ;  BEADS ;  WATER ;  ION ;  GEL
语种: 英语
WOS记录号: WOS:000266175100004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141300
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhang, Wei,Qin, Lei,He, Xi-Wen,et al. Novel surface modified molecularly imprinted polymer using acryloyl-beta-cyclodextrin and acrylamide as monomers for selective recognition of lysozyme in aqueous solution[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(21):4560-4567.
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