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题名: Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization
作者: Lin, Zian1, 2;  Yang, Fan1;  He, Xiwen1;  Zhang, Yukui1, 3
关键词: Covalent immobilization ;  Molecular imprinting ;  Organic-inorganic hybrid ;  Protein ;  Sol-gel
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-11-01
DOI: 10.1002/jssc.200900295
卷: 32, 期:22, 页:3980-3987
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The synthesis of poly-aminophenylboronic acid (APBA) imprinted hybrid silica-based polymers for selective recognition of bovine hemoglobin (BHb) was described, where the mesoporous hybrid silica supporting matrices were prepared by a mild sol-gel process with tetraethoxysilane and 3-aminopropyltriethoxysilane as two precursors. Covalent immobilization of BHb was adopted in order to create homogeneous recognition sites. After removal of the template, the resulting imprinted polymers showed high binding affinity toward BHb and the imprinting factor (alpha) reached 2.12. The specificity of the BHb recognition was evaluated with competitive experiments, indicating the imprinted polymers have a higher selectivity for the template BHb. The easy preparation protocol and good protein recognition proper-ties made the approach an attractive solution to depletion of high-abundance protein from bovine blood.
关键词[WOS]: MOLECULARLY IMPRINTED POLYMERS ;  SOLID-PHASE EXTRACTION ;  SERUM-ALBUMIN ;  THIN-FILMS ;  CATALYSIS ;  SURFACES
语种: 英语
WOS记录号: WOS:000273993500016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141341
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
2.Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian, Peoples R China

Recommended Citation:
Lin, Zian,Yang, Fan,He, Xiwen,et al. Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(22):3980-3987.
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