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Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization
Lin, Zian1,2; Yang, Fan1; He, Xiwen1; Zhang, Yukui1,3
关键词Covalent Immobilization Molecular Imprinting Organic-inorganic Hybrid Protein Sol-gel
刊名JOURNAL OF SEPARATION SCIENCE
2009-11-01
DOI10.1002/jssc.200900295
32期:22页:3980-3987
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MOLECULARLY IMPRINTED POLYMERS ; SOLID-PHASE EXTRACTION ; SERUM-ALBUMIN ; THIN-FILMS ; CATALYSIS ; SURFACES
英文摘要The synthesis of poly-aminophenylboronic acid (APBA) imprinted hybrid silica-based polymers for selective recognition of bovine hemoglobin (BHb) was described, where the mesoporous hybrid silica supporting matrices were prepared by a mild sol-gel process with tetraethoxysilane and 3-aminopropyltriethoxysilane as two precursors. Covalent immobilization of BHb was adopted in order to create homogeneous recognition sites. After removal of the template, the resulting imprinted polymers showed high binding affinity toward BHb and the imprinting factor (alpha) reached 2.12. The specificity of the BHb recognition was evaluated with competitive experiments, indicating the imprinted polymers have a higher selectivity for the template BHb. The easy preparation protocol and good protein recognition proper-ties made the approach an attractive solution to depletion of high-abundance protein from bovine blood.
语种英语
WOS记录号WOS:000273993500016
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被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141341
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
2.Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian, Peoples R China
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Lin, Zian,Yang, Fan,He, Xiwen,et al. Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(22):3980-3987.
APA Lin, Zian,Yang, Fan,He, Xiwen,&Zhang, Yukui.(2009).Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization.JOURNAL OF SEPARATION SCIENCE,32(22),3980-3987.
MLA Lin, Zian,et al."Organic-inorganic hybrid silica as supporting matrices for selective recognition of bovine hemoglobin via covalent immobilization".JOURNAL OF SEPARATION SCIENCE 32.22(2009):3980-3987.
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