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题名: Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template
作者: Hua, Zhendong1;  Zhou, Shuang1;  Zhao, Meiping1
关键词: Surface imprinting ;  Hydrogel ;  Bovine serum albumin ;  Silica microsphere
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2009-11-15
DOI: 10.1016/j.bios.2009.01.027
卷: 25, 期:3, 页:615-622
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: Surface imprinting is an effective approach to improve the template transfer efficiency in applications of molecularly imprinted polymers as biosensors and separation materials. In this paper, we tried to fabricate a surface imprinted hydrogel over silica microspheres for selective recognition of bovine serum albumin by covalent immobilization of a water-soluble UV sensitive initiator onto the surface of silica beads. The polymerization was initiated by UV radiation with N-[3-(dimethylamino)propyl]methacrylamide and N-isopropylacrylamide as the functional monomer and assistant monomer, respectively, and a thin coat Of stimuli-responsive hydrogel yielded over the silica gels. The surface imprinted hydrogels exhibited specific affinity toward the template protein with an association constant (K(a)) of 2.2 x 10(5) Lmol(-1) and a maximum binding capacity (Q(max)) of 27.3 mg g(-1) in Tris-HCl buffer (pH 7.0). The rebinding and desorption kinetics of the surface imprinted hydrogels were determined and proven to be extremely fast (about 1 min compared to 3 h for the previously prepared bulk imprinted hydrogel). Besides, the hydrogel-silica core-shell particles inherit both the stimuli-responsive property of the hydrogel and the good mechanical strength of the silica beads based on the on-line evaluation with high-performance liquid chromatography. The above comprehensive merits of the obtained surface imprinted hydrogel suggest the presented approach an attractive and broadly applicable way of developing biosensors and high-performance protein separation materials. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: POLYACRYLAMIDE-GEL BEADS ;  MINIEMULSION POLYMERIZATION ;  POLYMERS ;  RECOGNITION ;  IRRADIATION ;  INITIATORS ;  PARTICLES ;  LYSOZYME ;  CYSTEINE ;  FILMS
语种: 英语
WOS记录号: WOS:000271931900014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141342
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Hua, Zhendong,Zhou, Shuang,Zhao, Meiping. Fabrication of a surface imprinted hydrogel shell over silica microspheres using bovine serum albumin as a model protein template[J]. BIOSENSORS & BIOELECTRONICS,2009,25(3):615-622.
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