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题名: Simultaneous derivatization and extraction of free cyanide in biological samples with home-made hollow fiber-protected headspace liquid-phase microextraction followed by capillary electrophoresis with UV detection
作者: Meng, Liang1;  Liu, Xin2;  Wang, Bin2;  Shen, Guijun1;  Wang, Zhengqiao2;  Guo, Ming1, 3
关键词: Headspace liquid-phase microextraction ;  Home-made hollow fiber membrane ;  Free cyanide ;  Capillary electrophoresis ;  Urine ;  Saliva
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2009-11-01
DOI: 10.1016/j.jchromb.2009.09.006
卷: 877, 期:29, 页:3645-3651
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new method is reported for the simultaneous derivatization and extraction of free cyanide in biological samples using home-made hollow fiber-protected headspace liquid-phase microextraction (HF-HS-LPME) followed by capillary electrophoresis (CE) determination. The acceptor phase containing Ni(II)-NH(3) (as derivatization agent), sodium carbonate and ammonium pyromellitate (as internal standard) is held within a hollow fiber membrane, affixed to a syringe needle and immersed in the headspace of sample container. The extracted cyanide from the neutral samples forms a stable Ni(CN)(4)(2-) complex which is determined by CE. Parameters affecting extraction efficiency were investigated and optimized. For optimum peak shapes, the capillary was coated with cetyltrimethylammonium bromide (CTAB). The calibration Curve was linear for concentrations of CN(-) in the range from 0.1 to 20 mu mol L(-1) (R(2) = 0.9987). The LOD (S/N = 3) was estimated to be 0.01 mu mol L(-1) of CN(-) concentration. Such a detection sensitivity is high enough for free cyanide determination in common environmental and biological samples. Excellent repeatability of the extraction (RSD <= 5.6%, n = 5) was achieved. The feasibility of this method was demonstrated by analyzing human Urine and saliva samples with spiked recoveries in the range of 92-103.4%. This work provided an efficient alternative to the present headspace microextraction techniques Such as headspace solid-phase microextraction (HS-SPME) and headspace single-drop microextraction (HS-SDME). (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: SPECTROPHOTOMETRIC DETERMINATION ;  FLUORESCENCE DETECTION ;  SINGLE-DROP ;  WATER ;  ION ;  CHROMATOGRAPHY ;  BLOOD
语种: 英语
WOS记录号: WOS:000271168100024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141355
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
2.Dalian Publ Secur Bur, Forens Sci Inst, Dalian 116030, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116000, Peoples R China

Recommended Citation:
Meng, Liang,Liu, Xin,Wang, Bin,et al. Simultaneous derivatization and extraction of free cyanide in biological samples with home-made hollow fiber-protected headspace liquid-phase microextraction followed by capillary electrophoresis with UV detection[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(29):3645-3651.
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