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Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS
Yuan, Huiming1,2; Zhou, Yuan1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1
KeywordColumn Switch Recycling Size Exclusion Chromatography Immobilized Trypsin Microenzymatic Reactor Mu Rplc-esi-ms/ms Protein Analysis
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2009-10-30
DOI10.1016/j.chroma.2009.06.019
Volume1216Issue:44Pages:7478-7482
Indexed BySCI ; ISTP
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHUMAN PLASMA PROTEOME ; LIQUID-CHROMATOGRAPHY ; IDENTIFICATION ; ONLINE ; TECHNOLOGY ; DIGESTION
AbstractAn integrated platform with the combination of proteins and peptides separation was established via the unit of on-line proteins digestion, by which proteins were in sequence separated by column switch recycling size exclusion chromatography (csrSEC), on-line digested by an immobilized trypsin microreactor, trapped and desalted by two parallel C8 precolumns, separated by mu RPLC with the linear gradient of organic modifier concentration, and identified by ESI-MS/MS A 6-protein mixture. with Mr ranging from 10 kDa to 80 kDa. was used to evaluate the performance of the integrated platform, and all proteins were identified with sequence coverage over 5.67%. Our experimental results demonstrate that such an integrated platform is of advantages such as good time compatibility, high peak capacity, and facile automation. which might be a promising approach for proteome Study (C) 2009 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000271250500021
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141382
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Huiming,Zhou, Yuan,Zhang, Lihua,et al. Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(44):7478-7482.
APA Yuan, Huiming,Zhou, Yuan,Zhang, Lihua,Liang, Zhen,&Zhang, Yukui.(2009).Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS.JOURNAL OF CHROMATOGRAPHY A,1216(44),7478-7482.
MLA Yuan, Huiming,et al."Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS".JOURNAL OF CHROMATOGRAPHY A 1216.44(2009):7478-7482.
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