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Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS
Yuan, Huiming1,2; Zhou, Yuan1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1
关键词Column Switch Recycling Size Exclusion Chromatography Immobilized Trypsin Microenzymatic Reactor Mu Rplc-esi-ms/ms Protein Analysis
刊名JOURNAL OF CHROMATOGRAPHY A
2009-10-30
DOI10.1016/j.chroma.2009.06.019
1216期:44页:7478-7482
收录类别SCI ; ISTP
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HUMAN PLASMA PROTEOME ; LIQUID-CHROMATOGRAPHY ; IDENTIFICATION ; ONLINE ; TECHNOLOGY ; DIGESTION
英文摘要An integrated platform with the combination of proteins and peptides separation was established via the unit of on-line proteins digestion, by which proteins were in sequence separated by column switch recycling size exclusion chromatography (csrSEC), on-line digested by an immobilized trypsin microreactor, trapped and desalted by two parallel C8 precolumns, separated by mu RPLC with the linear gradient of organic modifier concentration, and identified by ESI-MS/MS A 6-protein mixture. with Mr ranging from 10 kDa to 80 kDa. was used to evaluate the performance of the integrated platform, and all proteins were identified with sequence coverage over 5.67%. Our experimental results demonstrate that such an integrated platform is of advantages such as good time compatibility, high peak capacity, and facile automation. which might be a promising approach for proteome Study (C) 2009 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000271250500021
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141382
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Yuan, Huiming,Zhou, Yuan,Zhang, Lihua,et al. Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(44):7478-7482.
APA Yuan, Huiming,Zhou, Yuan,Zhang, Lihua,Liang, Zhen,&Zhang, Yukui.(2009).Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS.JOURNAL OF CHROMATOGRAPHY A,1216(44),7478-7482.
MLA Yuan, Huiming,et al."Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS".JOURNAL OF CHROMATOGRAPHY A 1216.44(2009):7478-7482.
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