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题名: Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS
作者: Yuan, Huiming1, 2;  Zhou, Yuan1, 2;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
关键词: Column switch recycling size exclusion chromatography ;  Immobilized trypsin microenzymatic reactor ;  mu RPLC-ESI-MS/MS ;  Protein analysis
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-10-30
DOI: 10.1016/j.chroma.2009.06.019
卷: 1216, 期:44, 页:7478-7482
收录类别: SCI ;  ISTP
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: An integrated platform with the combination of proteins and peptides separation was established via the unit of on-line proteins digestion, by which proteins were in sequence separated by column switch recycling size exclusion chromatography (csrSEC), on-line digested by an immobilized trypsin microreactor, trapped and desalted by two parallel C8 precolumns, separated by mu RPLC with the linear gradient of organic modifier concentration, and identified by ESI-MS/MS A 6-protein mixture. with Mr ranging from 10 kDa to 80 kDa. was used to evaluate the performance of the integrated platform, and all proteins were identified with sequence coverage over 5.67%. Our experimental results demonstrate that such an integrated platform is of advantages such as good time compatibility, high peak capacity, and facile automation. which might be a promising approach for proteome Study (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: HUMAN PLASMA PROTEOME ;  LIQUID-CHROMATOGRAPHY ;  IDENTIFICATION ;  ONLINE ;  TECHNOLOGY ;  DIGESTION
语种: 英语
WOS记录号: WOS:000271250500021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141382
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Yuan, Huiming,Zhou, Yuan,Zhang, Lihua,et al. Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(44):7478-7482.
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