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题名: Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus
作者: Li, Xiang1;  Xu, Zhiliang;  Lu, Xin1;  Yang, Xuehui2;  Yin, Peiyuan1;  Kong, Hongwei1;  Yu, Ying3;  Xu, Guowang1
关键词: Metabonomics ;  Metabolomics ;  GC x GC-TOFMS ;  Biomarker ;  Diabetes mellitus
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2009-02-09
DOI: 10.1016/j.aca.2008.11.058
卷: 633, 期:2, 页:257-262
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC x GC-TOFMS) coupled with pattern recognition methods was applied to analyze plasma from diabetic patients and healthy controls. After sample preparation and GC x GC-TCFMS analysis, collected data were transformed, the peak alignment between different chromatograms was performed to generate the metabolites' peak table, then orthogonal signal correction filtered partial least-squares discriminant analysis (OSC-PLSDA) was carried out to model the data and discover metabolites with a significant concentration change in diabetic patients. With the method above, diabetic patients and healthy controls could be correctly distinguished based on the metabolic abnormity in plasma. Five potential biomarkers including glucose, 2-hydroxyisobutyric acid, linoleic acid, palmitic acid and phosphate were identified. It was found that elevated free fatty acids were essential pathophysiological factors in diabetes mellitus which reflected either the hyperglycemia or the deregulation of fatty acids metabolism. These potential biomarkers in plasma, e.g. palmitic acid, linoleic acid and 2-hydroxybutyric acid might be helpful in the diagnosis or further study of diabetes mellitus. This study shows the practicability and advantage of GC x GC-TOFMS coupled with data analysis and mining for metabonomics in biomarker discovery. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: X GC-TOFMS ;  FATTY-ACID CYCLE ;  INSULIN-RESISTANCE ;  TISSUE-EXTRACTS ;  METABOLITES ;  PLASMA ;  METABOLOMICS ;  DECONVOLUTION ;  PERFORMANCE ;  PLATFORM
语种: 英语
WOS记录号: WOS:000263188400014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141388
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Zhejiang Univ Tradit Chinese Med, Affiliated Hosp 1, Res Ctr Clin Lab, Hangzhou 310006, Zhejiang, Peoples R China
3.Zhejiang Tradit Chinese Med Univ, Affiliated Hosp 1, Dept Endocrinol, Hangzhou 310006, Zhejiang, Peoples R China

Recommended Citation:
Li, Xiang,Xu, Zhiliang,Lu, Xin,et al. Comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry for metabonomics: Biomarker discovery for diabetes mellitus[J]. ANALYTICA CHIMICA ACTA,2009,633(2):257-262.
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