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题名: Metabolic profiling of transgenic rice with cryIAc and sck genes: An evaluation of unintended effects at metabolic level by using GC-FID and GC-MS
作者: Zhou, Jia1;  Ma, Chenfei1;  Xu, Honglin2;  Yuan, Kailong1;  LU, Xin1;  Zhu, Zhen2;  Wu, Yongning3;  Xu, Guowang1
关键词: cryiac and sck genes ;  Metabolic profiling ;  Unintended effect ;  GC-FID ;  GC-MS ;  Transgenic rice ;  Environmental effects
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2009-03-15
DOI: 10.1016/j.jchromb.2009.01.040
卷: 877, 期:8-9, 页:725-732
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The crylAc and sck genes were introduced to the rice for the purpose of improving the insect resistance. Metabolic profiles of wild and transgenic rice were compared to assess the unintended effects related to gene modification. Wild samples with different sowing dates or sites were also examined to determine the environmental effects on metabolites. The polar compounds of grains were extracted, trimethylsilylated and analyzed by gas chromatography-flame ionization detection (GC-FID). Partial least squares-discriminant analysis (PLS-DA) and principal component analysis (PCA) were applied to differentiate transgenic and wild rice grains. The significantly distinguishable metabolites were picked out, and then identified by gas chromatography-mass spectrometry (GC-MS). It was found that both the environment and gene manipulation had remarkable impacts on the contents of glycerol-3-phosphate, citric acid, linoleic acid, oleic acid, hexadecanoic acid, 2,3-dihydroxypropyl ester, sucrose, 9-octadecenoic acid (Z)-, 2.3-dihydroxypropyl ester and so on. Sucrose, mannitol and glutamic acid had a significant increase in transgenic grains in contrast to those in non-genetically modified (GM) rice. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: CHROMATOGRAPHY-MASS SPECTROMETRY ;  GENETICALLY-MODIFIED FOODS ;  BACILLUS-THURINGIENSIS ;  NONTRANSGENIC COUNTERPART ;  DELTA-ENDOTOXIN ;  COMPLEMENTARY TOOL ;  SAFETY EVALUATION ;  ORYZA-SATIVA ;  BAR GENE ;  RESISTANCE
语种: 英语
WOS记录号: WOS:000264574300009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141409
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China
3.Chinese CDC, Natl Inst Nutr & Food Safety, Beijing 100050, Peoples R China

Recommended Citation:
Zhou, Jia,Ma, Chenfei,Xu, Honglin,et al. Metabolic profiling of transgenic rice with cryIAc and sck genes: An evaluation of unintended effects at metabolic level by using GC-FID and GC-MS[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(8-9):725-732.
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