中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Microchip free flow isoelectric focusing for protein prefractionation using monolith with immobilized pH gradient
作者: Han, Bin1, 2;  Wang, Pingli1;  Zhu, Guijie1;  Zhang, Lihua1;  Qu, Feng2;  Deng, Yulin2;  Zhang, Yukui1
关键词: Free flow electrophoresis ;  IEF ;  IPG ;  Microchip ;  Monolithic materials
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-04-01
DOI: 10.1002/jssc.200800572
卷: 32, 期:8, 页:1211-1215
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Microchip free flow IEF (mu FFIEF) with monolithic IPG was proposed for protein prefractionation. The monolithic materials were first prepared by UV irradiation in a microchamber of 4:3 mm length, 23 mm width, and 38 mu m depth. Carrier ampholytes (CAs) were further immobilized on the monolith by chemical bonding, to form a stable pH gradient. BY such a technique, the continuous introduction of CAS ill traditional mu FFIEF could be avoided, not only to decrease the operation cost, but also to avoid the interference of CAs for the further protein identification by MS/MS. With a fluorescence microscope as the detector, under the optimal conditions, the separation of FITC labeled beta-lactoglobulin and carbonic anhydrase. with 0.9 unit difference on pl, was achieved with good reproducibility. The experimental results demonstrated that under the experimental conditions we applied, the separation mechanism of mu FFIEF with M-TPG materials might be the cooperative effects of IEF and CZE, and the former one plays a predominant role.
关键词[WOS]: ELECTROPHORESIS ;  DEVICE ;  RESOLUTION ;  COLUMN ;  SPEED ;  CHIP
语种: 英语
WOS记录号: WOS:000265793700012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141433
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China

Recommended Citation:
Han, Bin,Wang, Pingli,Zhu, Guijie,et al. Microchip free flow isoelectric focusing for protein prefractionation using monolith with immobilized pH gradient[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(8):1211-1215.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Han, Bin]'s Articles
 [Wang, Pingli]'s Articles
 [Zhu, Guijie]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Han, Bin]‘s Articles
 [Wang, Pingli]‘s Articles
 [Zhu, Guijie]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace