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题名: BSA-imprinted synthetic receptor for reversible template recognition
作者: Wang, Huafang1;  He, Yunhua1;  He, Xiwen1;  Li, Wenyou1;  Chen, Langxing1;  Zhang, Yukui1, 2
关键词: Bovine serum albumin (BSA) ;  Chitosan ;  High performance liquid chromatography (HPLC) ;  Molecularly imprinted polymers (MIPs)
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-06-01
DOI: 10.1002/jssc.200800562
卷: 32, 期:11, 页:1981-1986
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A novel approach to the manufacturing of protein-responsive imprints on a home-made chitosan substrate was established together with m-aminophenylboronic acid (APBA) as a functional monomer. The produced polymers were characterized using both (1) equilibrium adsorption assays and (2) high performance liquid chromatography analysis. Results confirmed that the synthesized BSA-MIP (molecularly imprinted polymer) has a high affinity towards its template compared to the determined control proteins. The produced BSA-MIP featured largely in its good adsorption reversibility, especially in competitive binding assays, which is of great biological significance in separations. Non-specific binding was reduced to almost zero in a BSA/BHb competitive binding event. An excellent HPLC profile of template recognition was found for BSA-MIP, even under harsh mobile phase conditions. In the present work, the adopted trapped-template-release method permits recovery of bound BSA [1]. The strategy of making an artificial protein-receptor with high adsorption affinity and reversibility is promising in on-line isolation of target protein from complicated biological environments.
关键词[WOS]: BOVINE SERUM-ALBUMIN ;  3-AMINOPHENYLBORONIC ACID ;  FUNCTIONAL MONOMER ;  THIN-FILMS ;  SURFACE ;  POLYMER ;  PROTEINS
语种: 英语
WOS记录号: WOS:000266929700024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141452
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Wang, Huafang,He, Yunhua,He, Xiwen,et al. BSA-imprinted synthetic receptor for reversible template recognition[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(11):1981-1986.
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