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BSA-imprinted synthetic receptor for reversible template recognition
Wang, Huafang1; He, Yunhua1; He, Xiwen1; Li, Wenyou1; Chen, Langxing1; Zhang, Yukui1,2
关键词Bovine Serum Albumin (Bsa) Chitosan High Performance Liquid Chromatography (Hplc) Molecularly Imprinted Polymers (Mips)
刊名JOURNAL OF SEPARATION SCIENCE
2009-06-01
DOI10.1002/jssc.200800562
32期:11页:1981-1986
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]BOVINE SERUM-ALBUMIN ; 3-AMINOPHENYLBORONIC ACID ; FUNCTIONAL MONOMER ; THIN-FILMS ; SURFACE ; POLYMER ; PROTEINS
英文摘要A novel approach to the manufacturing of protein-responsive imprints on a home-made chitosan substrate was established together with m-aminophenylboronic acid (APBA) as a functional monomer. The produced polymers were characterized using both (1) equilibrium adsorption assays and (2) high performance liquid chromatography analysis. Results confirmed that the synthesized BSA-MIP (molecularly imprinted polymer) has a high affinity towards its template compared to the determined control proteins. The produced BSA-MIP featured largely in its good adsorption reversibility, especially in competitive binding assays, which is of great biological significance in separations. Non-specific binding was reduced to almost zero in a BSA/BHb competitive binding event. An excellent HPLC profile of template recognition was found for BSA-MIP, even under harsh mobile phase conditions. In the present work, the adopted trapped-template-release method permits recovery of bound BSA [1]. The strategy of making an artificial protein-receptor with high adsorption affinity and reversibility is promising in on-line isolation of target protein from complicated biological environments.
语种英语
WOS记录号WOS:000266929700024
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141452
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
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GB/T 7714
Wang, Huafang,He, Yunhua,He, Xiwen,et al. BSA-imprinted synthetic receptor for reversible template recognition[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(11):1981-1986.
APA Wang, Huafang,He, Yunhua,He, Xiwen,Li, Wenyou,Chen, Langxing,&Zhang, Yukui.(2009).BSA-imprinted synthetic receptor for reversible template recognition.JOURNAL OF SEPARATION SCIENCE,32(11),1981-1986.
MLA Wang, Huafang,et al."BSA-imprinted synthetic receptor for reversible template recognition".JOURNAL OF SEPARATION SCIENCE 32.11(2009):1981-1986.
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