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BSA-imprinted synthetic receptor for reversible template recognition
Wang, Huafang1; He, Yunhua1; He, Xiwen1; Li, Wenyou1; Chen, Langxing1; Zhang, Yukui1,2
KeywordBovine Serum Albumin (Bsa) Chitosan High Performance Liquid Chromatography (Hplc) Molecularly Imprinted Polymers (Mips)
Source PublicationJOURNAL OF SEPARATION SCIENCE
2009-06-01
DOI10.1002/jssc.200800562
Volume32Issue:11Pages:1981-1986
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordBOVINE SERUM-ALBUMIN ; 3-AMINOPHENYLBORONIC ACID ; FUNCTIONAL MONOMER ; THIN-FILMS ; SURFACE ; POLYMER ; PROTEINS
AbstractA novel approach to the manufacturing of protein-responsive imprints on a home-made chitosan substrate was established together with m-aminophenylboronic acid (APBA) as a functional monomer. The produced polymers were characterized using both (1) equilibrium adsorption assays and (2) high performance liquid chromatography analysis. Results confirmed that the synthesized BSA-MIP (molecularly imprinted polymer) has a high affinity towards its template compared to the determined control proteins. The produced BSA-MIP featured largely in its good adsorption reversibility, especially in competitive binding assays, which is of great biological significance in separations. Non-specific binding was reduced to almost zero in a BSA/BHb competitive binding event. An excellent HPLC profile of template recognition was found for BSA-MIP, even under harsh mobile phase conditions. In the present work, the adopted trapped-template-release method permits recovery of bound BSA [1]. The strategy of making an artificial protein-receptor with high adsorption affinity and reversibility is promising in on-line isolation of target protein from complicated biological environments.
Language英语
WOS IDWOS:000266929700024
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141452
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Wang, Huafang,He, Yunhua,He, Xiwen,et al. BSA-imprinted synthetic receptor for reversible template recognition[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(11):1981-1986.
APA Wang, Huafang,He, Yunhua,He, Xiwen,Li, Wenyou,Chen, Langxing,&Zhang, Yukui.(2009).BSA-imprinted synthetic receptor for reversible template recognition.JOURNAL OF SEPARATION SCIENCE,32(11),1981-1986.
MLA Wang, Huafang,et al."BSA-imprinted synthetic receptor for reversible template recognition".JOURNAL OF SEPARATION SCIENCE 32.11(2009):1981-1986.
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