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"One-Pot" Process for Fabrication of Organic-Silica Hybrid Monolithic Capillary Columns Using Organic Monomer and Alkoxysilane
Wu, Minghuo1; Wu, Ren'an1; Wang, Fangjun1; Ren, Lianbing2; Dong, Jing1; Liu, Zhen2; Zou, Hanfa1
Source PublicationANALYTICAL CHEMISTRY
2009-05-01
DOI10.1021/ac9000749
Volume81Issue:9Pages:3529-3536
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; STATIONARY PHASES ; PROTEOME ANALYSIS ; HIGH-EFFICIENCY ; ELECTROCHROMATOGRAPHY ; IMPROVEMENT ; SEPARATION ; FLOW
AbstractA "one-pot" process for the preparation of organic-silica hybrid capillary monolithic columns by concurrently using organic monomers and alkoxysilanes was described. In this process, the hydrolyzed alkoxysilanes of tetramethoxysilane (TMOS) and vinyltrimethoxysilane (VTMS) as precursors for the synthesis of a silica.-based monolith using the sol-gel method and the organic monomer (allyldimethyldodecylammonium bromide (ADDAB) or acrylamide (AA)) with vinyl groups for free radical polymerization along with the initiator of azobisisobutyronitrile (AIBN) were concurrently introduced into a pretreated capillary; after that, the polycondensation of alkoxysilanes and the copolymerization of organic monomers and as-precondensed siloxanes were subsequently carried out within the confines of a capillary at the proper reaction conditions. Two types of organic-silica hybrid capillary monolithic columns with hydrophobic and hydrophilic properties have been fabricated, respectively, by this "onepot" process using two different organic monomers of ADDAB and AA. The morphologies of the synthesized organic-silica hybrid monolithic columns were characterized by scanning electron microscopy (SEIM). The performances of these organic-silica monolithic columns were investigated by capillary electrochromatography (CEC). The retention behaviors of the neutral and polar compounds on the resulting hydrophobic and hydrophilic organic-silica hybrid monolithic columns confirmed the successful incorporation of organic monomers in the silica monolithic matrix. In addition, the JWDA-silica hybrid capillary monolithic column was also applied in the analysis of tryptic-digests of bovine serum albumin (BSA) and mouse liver extract by microliquid chromatography-tandem mass spectrometry (mu LC-MS/MS) for demonstrating its potential in proteome analysis.
Language英语
WOS IDWOS:000265632400045
Citation statistics
Cited Times:112[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141457
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China
Recommended Citation
GB/T 7714
Wu, Minghuo,Wu, Ren'an,Wang, Fangjun,et al. "One-Pot" Process for Fabrication of Organic-Silica Hybrid Monolithic Capillary Columns Using Organic Monomer and Alkoxysilane[J]. ANALYTICAL CHEMISTRY,2009,81(9):3529-3536.
APA Wu, Minghuo.,Wu, Ren'an.,Wang, Fangjun.,Ren, Lianbing.,Dong, Jing.,...&Zou, Hanfa.(2009)."One-Pot" Process for Fabrication of Organic-Silica Hybrid Monolithic Capillary Columns Using Organic Monomer and Alkoxysilane.ANALYTICAL CHEMISTRY,81(9),3529-3536.
MLA Wu, Minghuo,et al.""One-Pot" Process for Fabrication of Organic-Silica Hybrid Monolithic Capillary Columns Using Organic Monomer and Alkoxysilane".ANALYTICAL CHEMISTRY 81.9(2009):3529-3536.
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