DICP OpenIR
Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice
Ding, Wenyong1,2,3; Lin, Lin2; Ren, Feng2; Zou, Hanfa; Duan, Ziyuan1; Dai, Jianwu1
关键词Histamine H-3 Receptor (H3r) Alternative Splicing Intron Retention Splice Site Minigene
刊名JOURNAL OF GENETICS AND GENOMICS
2009-08-01
DOI10.1016/S1673-8527(08)60137-X
36期:8页:475-482
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]OVERLAP EXTENSION ; ISOFORMS ; GENE ; CLONING ; ORGANIZATION ; EXPRESSION ; PCR ; RECOGNITION ; MICROARRAYS ; MUTAGENESIS
英文摘要In the alternative splicing, intron retention, of histamine H-3 receptors in rats and mice, the short transcript isoforms that are excised alternatively spliced introns are easily detected in a very low level in rats and arc undetectable in mice using the regular PCR protocol. The retained introns have common 5' splice site and different 3' splice sites. The detailed mechanism for the special alternative splicing remains largely unclear. In this study, we developed a minigene splicing system to recapitulate natural alternative splicing of the receptors and investigated the effects of 5' and 3' splice sites on intron retention in HeLa cells. Mutating weak 5' and 3' splice sites of the alternatively spliced introns toward the canonical consensus sequences promoted the splicing of the corresponding introns in rat and mouse minigenes. The effect of splice site strength was context-dependent and much more significant for the 3' splice site of the longer alternative intron than for the 3' splice site of the shorter alternative intron and the common 5' splice sites; it was also more significant in the rat minigene than in the mouse minigene. Mutating the 3' splice site of the longer alternative intron resulted in almost complete splicing of the intron and made the corresponding isoform to become the nearly exclusive transcript in the rat minigene.
语种英语
WOS记录号WOS:000268980800004
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141464
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China
2.Dalian Med Univ, Dept Biochem & Mol Biol, Dalian 116044, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Ding, Wenyong,Lin, Lin,Ren, Feng,et al. Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice[J]. JOURNAL OF GENETICS AND GENOMICS,2009,36(8):475-482.
APA Ding, Wenyong,Lin, Lin,Ren, Feng,Zou, Hanfa,Duan, Ziyuan,&Dai, Jianwu.(2009).Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice.JOURNAL OF GENETICS AND GENOMICS,36(8),475-482.
MLA Ding, Wenyong,et al."Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice".JOURNAL OF GENETICS AND GENOMICS 36.8(2009):475-482.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ding, Wenyong]的文章
[Lin, Lin]的文章
[Ren, Feng]的文章
百度学术
百度学术中相似的文章
[Ding, Wenyong]的文章
[Lin, Lin]的文章
[Ren, Feng]的文章
必应学术
必应学术中相似的文章
[Ding, Wenyong]的文章
[Lin, Lin]的文章
[Ren, Feng]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。