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题名: Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice
作者: Ding, Wenyong1, 2, 3;  Lin, Lin2;  Ren, Feng2;  Zou, Hanfa;  Duan, Ziyuan1;  Dai, Jianwu1
关键词: histamine H-3 receptor (H3R) ;  alternative splicing ;  intron retention ;  splice site ;  minigene
刊名: JOURNAL OF GENETICS AND GENOMICS
发表日期: 2009-08-01
DOI: 10.1016/S1673-8527(08)60137-X
卷: 36, 期:8, 页:475-482
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ;  Genetics & Heredity
英文摘要: In the alternative splicing, intron retention, of histamine H-3 receptors in rats and mice, the short transcript isoforms that are excised alternatively spliced introns are easily detected in a very low level in rats and arc undetectable in mice using the regular PCR protocol. The retained introns have common 5' splice site and different 3' splice sites. The detailed mechanism for the special alternative splicing remains largely unclear. In this study, we developed a minigene splicing system to recapitulate natural alternative splicing of the receptors and investigated the effects of 5' and 3' splice sites on intron retention in HeLa cells. Mutating weak 5' and 3' splice sites of the alternatively spliced introns toward the canonical consensus sequences promoted the splicing of the corresponding introns in rat and mouse minigenes. The effect of splice site strength was context-dependent and much more significant for the 3' splice site of the longer alternative intron than for the 3' splice site of the shorter alternative intron and the common 5' splice sites; it was also more significant in the rat minigene than in the mouse minigene. Mutating the 3' splice site of the longer alternative intron resulted in almost complete splicing of the intron and made the corresponding isoform to become the nearly exclusive transcript in the rat minigene.
关键词[WOS]: OVERLAP EXTENSION ;  ISOFORMS ;  GENE ;  CLONING ;  ORGANIZATION ;  EXPRESSION ;  PCR ;  RECOGNITION ;  MICROARRAYS ;  MUTAGENESIS
语种: 英语
WOS记录号: WOS:000268980800004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141464
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Mol Dev Biol, Beijing 100080, Peoples R China
2.Dalian Med Univ, Dept Biochem & Mol Biol, Dalian 116044, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Ding, Wenyong,Lin, Lin,Ren, Feng,et al. Effects of splice sites on the intron retention in histamine H-3 receptors from rats and mice[J]. JOURNAL OF GENETICS AND GENOMICS,2009,36(8):475-482.
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