中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes
作者: Guo, Weihua2;  Ye, Zhiqiang1;  Wang, Guilan1;  Zhao, Xiaoming2;  Yuan, Jingli1;  Du, Yuguang2
关键词: Oligochitosan ;  Tobacco cells ;  Interaction ;  Europium complex ;  Time-resolved fluorescence
刊名: TALANTA
发表日期: 2009-05-15
DOI: 10.1016/j.talanta.2009.01.020
卷: 78, 期:3, 页:977-982
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The interaction of oligochitosan and tobacco cells has been investigated by fluorometric method using two Eu(3+) complexes as the probes in this work. Based on the reaction of tobacco cells with oligochitosan conjugated to a strongly fluorescent Eu(3+) complex 4,4'-bis(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl)chlorosulfo-o-terphenyl-Eu(3+) (oligochitosan-BHHCT-Eu(3+) conjugate), the binding kinetic process of oligochitosan-tobacco cells was fluorescently imaged. The results indicate that oligochitosan can be specifically bound to the walls as well as the membranes of tobacco cells. A sensitive and selective Eu(3+) complex luminescence probe specific for singlet oxygen, [4'-(10-methyl-9-anthryl)-2,2':6',2 ''-terpyridine-6,6 ''-diyl]bis(methylenenitrilo)tetrakis (acetate)-Eu(3+), Was used for developing a new time-resolved fluorescence assay method for the determinations of indole-3-acetic acid (IAA) and peroxidase produced in the cells during the interaction of oligochitosan and tobacco cells. The assays are sensitive with the detection limits of 32 nM for IAA, and 1.2 nM for peroxidase, respectively. The concentration changes of IAA and peroxidase induced by oligochitosan in tobacco cells reveal that oligochitosan can effectively induce the increase of IAA concentration, accompanied by the decrease of peroxidase concentration. These results give a primary and reliable evidence to explain the growth-promoting mechanism of oligochitosan on the plants at molecular level. (c) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: TIME-RESOLVED FLUOROIMMUNOASSAY ;  CATALYZED AEROBIC OXIDATION ;  SINGLET OXYGEN PRODUCTION ;  ENERGY-TRANSFER ;  IN-VITRO ;  RESISTANCE ;  ACID ;  CHITOOLIGOSACCHARIDES ;  CHITOSAN ;  ENZYME
语种: 英语
WOS记录号: WOS:000264976600055
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141466
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Guo, Weihua,Ye, Zhiqiang,Wang, Guilan,et al. Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes[J]. TALANTA,2009,78(3):977-982.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Guo, Weihua]'s Articles
 [Ye, Zhiqiang]'s Articles
 [Wang, Guilan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Guo, Weihua]‘s Articles
 [Ye, Zhiqiang]‘s Articles
 [Wang, Guilan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace