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Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes
Guo, Weihua2; Ye, Zhiqiang1; Wang, Guilan1; Zhao, Xiaoming2; Yuan, Jingli1; Du, Yuguang2
KeywordOligochitosan Tobacco Cells Interaction Europium Complex Time-resolved Fluorescence
Source PublicationTALANTA
2009-05-15
DOI10.1016/j.talanta.2009.01.020
Volume78Issue:3Pages:977-982
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTIME-RESOLVED FLUOROIMMUNOASSAY ; CATALYZED AEROBIC OXIDATION ; SINGLET OXYGEN PRODUCTION ; ENERGY-TRANSFER ; IN-VITRO ; RESISTANCE ; ACID ; CHITOOLIGOSACCHARIDES ; CHITOSAN ; ENZYME
AbstractThe interaction of oligochitosan and tobacco cells has been investigated by fluorometric method using two Eu(3+) complexes as the probes in this work. Based on the reaction of tobacco cells with oligochitosan conjugated to a strongly fluorescent Eu(3+) complex 4,4'-bis(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 ''-yl)chlorosulfo-o-terphenyl-Eu(3+) (oligochitosan-BHHCT-Eu(3+) conjugate), the binding kinetic process of oligochitosan-tobacco cells was fluorescently imaged. The results indicate that oligochitosan can be specifically bound to the walls as well as the membranes of tobacco cells. A sensitive and selective Eu(3+) complex luminescence probe specific for singlet oxygen, [4'-(10-methyl-9-anthryl)-2,2':6',2 ''-terpyridine-6,6 ''-diyl]bis(methylenenitrilo)tetrakis (acetate)-Eu(3+), Was used for developing a new time-resolved fluorescence assay method for the determinations of indole-3-acetic acid (IAA) and peroxidase produced in the cells during the interaction of oligochitosan and tobacco cells. The assays are sensitive with the detection limits of 32 nM for IAA, and 1.2 nM for peroxidase, respectively. The concentration changes of IAA and peroxidase induced by oligochitosan in tobacco cells reveal that oligochitosan can effectively induce the increase of IAA concentration, accompanied by the decrease of peroxidase concentration. These results give a primary and reliable evidence to explain the growth-promoting mechanism of oligochitosan on the plants at molecular level. (c) 2009 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000264976600055
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141466
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Dept Chem, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Guo, Weihua,Ye, Zhiqiang,Wang, Guilan,et al. Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes[J]. TALANTA,2009,78(3):977-982.
APA Guo, Weihua,Ye, Zhiqiang,Wang, Guilan,Zhao, Xiaoming,Yuan, Jingli,&Du, Yuguang.(2009).Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes.TALANTA,78(3),977-982.
MLA Guo, Weihua,et al."Measurement of oligochitosan-tobacco cell interaction by fluorometric method using europium complexes as fluorescence probes".TALANTA 78.3(2009):977-982.
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