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题名: Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene
作者: Li, Huihui1, 2;  Liu, Yiquan1;  Lin, Sen1;  Yuan, Gu1
关键词: DNA structures ;  duplexes ;  G-quadruplexes ;  noncovalent interactions ;  oncogenes
刊名: CHEMISTRY-A EUROPEAN JOURNAL
发表日期: 2009
DOI: 10.1002/chem.200801922
卷: 15, 期:10, 页:2445-2452
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: This study has demonstrated the formation of the G-quadruplex structure from the G-rich sequence in the promoter region of the bcl-2 oncogene; the formation could be induced by addition of NH(4)(+) or K(+) ions. The binding affinity and stoichiometry of seven Small Molecules With the G-quadruplex were examined by using F,SI-MS. its well as CD and UV spectroscopy. The binding-affinity order was determined to be P1 approximate to P5 > P2 > P3 approximate to P4 > P7 > P6. In particular, the small-molecule induction of the structural transition between the G-quadruplex and duplex DNA forms in this promoter region was investigated by ESI-MS. We directly observed specific binding of dehydrocorydaline (P7) and cationic porphyrin (P5) in one system consisting of the G-quadruplex and the duplex DNA, respectively. The results indicate that P7 selectively stabilizes the G-quadruplex and shifts the equalibrium toward G-quadruplex formation of the bcl-2 promoter, whereas P5 converts the G-quadruplex into the duplex DNA, which results in strong and selective binding to the duplex form. Therefore, PS and P7 with their attractive binding specificities could be considered as precursors for pathway-specific drug, design for regulation of bcl-2 oncogene transcription.
关键词[WOS]: IONIZATION MASS-SPECTROMETRY ;  AUTOMATED DOCKING ;  SMALL-MOLECULE ;  DNA ;  BINDING ;  PROTEINS ;  DUPLEX ;  DISTAMYCIN ;  TRANSITION ;  EXPRESSION
语种: 英语
WOS记录号: WOS:000264029500026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141467
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Dept Chem Biol,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Nanjing Normal Univ, Jiangsu Key Lab Biofunct Mat, Sch Chem & Environm Sci, Nanjing 210097, Peoples R China

Recommended Citation:
Li, Huihui,Liu, Yiquan,Lin, Sen,et al. Spectroscopy Probing of the Formation, Recognition, and Conversion of a G-Quadruplex in the Promoter Region of the bcl-2 Oncogene[J]. CHEMISTRY-A EUROPEAN JOURNAL,2009,15(10):2445-2452.
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