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题名: Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance
作者: Wang Siyan2, 4;  Yue Feng3;  Zhang Lichuan4;  Wang Jiarui4;  Wang Yingyan5;  Jiang Li4;  Lin Bingcheng6;  Wang Qi1
关键词: Microfluidic gradient chip ;  Human lung squamous carcinoma ;  Glucose Regulated Protein-78 ;  Chemotherapy resistance ;  Apoptosis
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2009-04-05
DOI: 10.1016/j.jpba.2008.12.021
卷: 49, 期:3, 页:806-810
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: The major challenge of chemotherapy is the disease resistance for many lung cancer patients. Integrated microfluidic systems offer many desirable characteristics and can be used in cellular biological analysis. This work aimed to study the correlation between the expression of Glucose Regulated Protein-78 (GRP78) and the resistance to anticancer drug VP-16 in human lung squamous carcinoma cell line SK-MES-1 using an integrated microfluidic gradient chip device. We used A23187, a GRP78 inducer, with a gradient concentration in the upstream network of the device to induce the expression of GRP78 in the cells cultured in the downstream before the addition of VP-16. The expression of GRP78 was detected by immunofluorescence, the apoptosis for the cells treated by VP-16 was assessed morphologically by 4',6-diamidino-2-phenylindole (DAPI) staining. The results indicated that the expressions of GRP78 increased greatly for the cells under the induction of A23187 with a dose-depended manner, while the percentage of apoptotic cells decreased significantly after being treated by VP-16. Our results from this study confirmed the role of GRP78 played in the chemotherapy resistance to VP-16 in SK-MES-1 cell line, suggesting that the integrated microfluidic systems may be an unique approach for characterizing the cellular responses. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: TOTAL ANALYSIS SYSTEMS ;  INDUCED APOPTOSIS ;  CELL ;  MICROCHIP ;  DEVICE ;  INTEGRATION ;  MODULATION ;  GENERATION ;  NETWORKS
语种: 英语
WOS记录号: WOS:000264409800033
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141486
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Hosp Affiliated 2, Dept Resp Med, Dalian 116027, Peoples R China
2.Dalian Med Univ, Hosp Affiliated 2, Dept Emergencyt, Dalian 116027, Peoples R China
3.Dalian Municipal Friendship Hosp, Dept Urol, Dalian 116001, Peoples R China
4.Dalian Med Univ, Grad Sch, Dalian 116044, Peoples R China
5.Dalian Med Univ, Diagnost Lab Ctr, Dalian 116044, Peoples R China
6.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Wang Siyan,Yue Feng,Zhang Lichuan,et al. Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2009,49(3):806-810.
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