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Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance
Wang Siyan2,4; Yue Feng3; Zhang Lichuan4; Wang Jiarui4; Wang Yingyan5; Jiang Li4; Lin Bingcheng6; Wang Qi1
关键词Microfluidic Gradient Chip Human Lung Squamous Carcinoma Glucose Regulated Protein-78 Chemotherapy Resistance Apoptosis
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2009-04-05
DOI10.1016/j.jpba.2008.12.021
49期:3页:806-810
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]TOTAL ANALYSIS SYSTEMS ; INDUCED APOPTOSIS ; CELL ; MICROCHIP ; DEVICE ; INTEGRATION ; MODULATION ; GENERATION ; NETWORKS
英文摘要The major challenge of chemotherapy is the disease resistance for many lung cancer patients. Integrated microfluidic systems offer many desirable characteristics and can be used in cellular biological analysis. This work aimed to study the correlation between the expression of Glucose Regulated Protein-78 (GRP78) and the resistance to anticancer drug VP-16 in human lung squamous carcinoma cell line SK-MES-1 using an integrated microfluidic gradient chip device. We used A23187, a GRP78 inducer, with a gradient concentration in the upstream network of the device to induce the expression of GRP78 in the cells cultured in the downstream before the addition of VP-16. The expression of GRP78 was detected by immunofluorescence, the apoptosis for the cells treated by VP-16 was assessed morphologically by 4',6-diamidino-2-phenylindole (DAPI) staining. The results indicated that the expressions of GRP78 increased greatly for the cells under the induction of A23187 with a dose-depended manner, while the percentage of apoptotic cells decreased significantly after being treated by VP-16. Our results from this study confirmed the role of GRP78 played in the chemotherapy resistance to VP-16 in SK-MES-1 cell line, suggesting that the integrated microfluidic systems may be an unique approach for characterizing the cellular responses. (C) 2008 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000264409800033
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141486
专题中国科学院大连化学物理研究所
作者单位1.Dalian Med Univ, Hosp Affiliated 2, Dept Resp Med, Dalian 116027, Peoples R China
2.Dalian Med Univ, Hosp Affiliated 2, Dept Emergencyt, Dalian 116027, Peoples R China
3.Dalian Municipal Friendship Hosp, Dept Urol, Dalian 116001, Peoples R China
4.Dalian Med Univ, Grad Sch, Dalian 116044, Peoples R China
5.Dalian Med Univ, Diagnost Lab Ctr, Dalian 116044, Peoples R China
6.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Wang Siyan,Yue Feng,Zhang Lichuan,et al. Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2009,49(3):806-810.
APA Wang Siyan.,Yue Feng.,Zhang Lichuan.,Wang Jiarui.,Wang Yingyan.,...&Wang Qi.(2009).Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,49(3),806-810.
MLA Wang Siyan,et al."Application of microfluidic gradient chip in the analysis of lung cancer chemotherapy resistance".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 49.3(2009):806-810.
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