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Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications
Zhang Yu1,2; Yu Hao1; Dong Xiu-Ling3; Qin Jian-Hua1; Lin Bin-Cheng1
关键词Microfluidic Chip Acrylamide Hydrogel Dna Probes Immobilization Hybridization
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2009-06-10
30期:6页:1128-1130
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]ELECTROCHEMICAL BIOSENSOR ; HYBRIDIZATION ; ASSAY ; OLIGONUCLEOTIDE ; CAPTURE
英文摘要A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented. Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions, and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA. The total analysis process can be completed within 5 min, and the limit of detection is 0.1 mu mol/L. This method is simple, rapid and feasible. The double-stranded DNA can be chemically denatured, and the chip is reusable. The conditions for photopolymerization, hybridization, and denaturation were discussed as well.
语种英语
WOS记录号WOS:000267873700014
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141489
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China
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GB/T 7714
Zhang Yu,Yu Hao,Dong Xiu-Ling,et al. Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(6):1128-1130.
APA Zhang Yu,Yu Hao,Dong Xiu-Ling,Qin Jian-Hua,&Lin Bin-Cheng.(2009).Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,30(6),1128-1130.
MLA Zhang Yu,et al."Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 30.6(2009):1128-1130.
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