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题名: Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications
作者: Zhang Yu1, 2;  Yu Hao1;  Dong Xiu-Ling3;  Qin Jian-Hua1;  Lin Bin-Cheng1
关键词: Microfluidic chip ;  Acrylamide hydrogel ;  DNA probes immobilization ;  Hybridization
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2009-06-10
卷: 30, 期:6, 页:1128-1130
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented. Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions, and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA. The total analysis process can be completed within 5 min, and the limit of detection is 0.1 mu mol/L. This method is simple, rapid and feasible. The double-stranded DNA can be chemically denatured, and the chip is reusable. The conditions for photopolymerization, hybridization, and denaturation were discussed as well.
关键词[WOS]: ELECTROCHEMICAL BIOSENSOR ;  HYBRIDIZATION ;  ASSAY ;  OLIGONUCLEOTIDE ;  CAPTURE
语种: 英语
WOS记录号: WOS:000267873700014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141489
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China

Recommended Citation:
Zhang Yu,Yu Hao,Dong Xiu-Ling,et al. Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(6):1128-1130.
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