DICP OpenIR
Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications
Zhang Yu1,2; Yu Hao1; Dong Xiu-Ling3; Qin Jian-Hua1; Lin Bin-Cheng1
KeywordMicrofluidic Chip Acrylamide Hydrogel Dna Probes Immobilization Hybridization
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2009-06-10
Volume30Issue:6Pages:1128-1130
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordELECTROCHEMICAL BIOSENSOR ; HYBRIDIZATION ; ASSAY ; OLIGONUCLEOTIDE ; CAPTURE
AbstractA method of DNA probes immobilization and specific target sequences capture in a microfluidic chip was presented. Acrydite-modified DNA probes are immobilized in silanized glass microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions, and the immobilized DNA probes can be hybridized with fluorescence labeled complementary DNA. The total analysis process can be completed within 5 min, and the limit of detection is 0.1 mu mol/L. This method is simple, rapid and feasible. The double-stranded DNA can be chemically denatured, and the chip is reusable. The conditions for photopolymerization, hybridization, and denaturation were discussed as well.
Language英语
WOS IDWOS:000267873700014
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141489
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China
Recommended Citation
GB/T 7714
Zhang Yu,Yu Hao,Dong Xiu-Ling,et al. Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(6):1128-1130.
APA Zhang Yu,Yu Hao,Dong Xiu-Ling,Qin Jian-Hua,&Lin Bin-Cheng.(2009).Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,30(6),1128-1130.
MLA Zhang Yu,et al."Immobilization of DNA Hydrogel Plugs in Glass Microfluidic Channels and Its Applications".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 30.6(2009):1128-1130.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang Yu]'s Articles
[Yu Hao]'s Articles
[Dong Xiu-Ling]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang Yu]'s Articles
[Yu Hao]'s Articles
[Dong Xiu-Ling]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang Yu]'s Articles
[Yu Hao]'s Articles
[Dong Xiu-Ling]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.