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Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein
Pan, Qiong1; Zhao, Meiping1; Liu, Shaorong2
刊名ANALYTICAL CHEMISTRY
2009-07-01
DOI10.1021/ac9007607
81期:13页:5333-5341
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MICELLAR ELECTROKINETIC CHROMATOGRAPHY ; AMPLIFIED SAMPLE INJECTION ; ELECTROPHORESIS-MASS SPECTROMETRY ; SYSTEM CAPILLARY-ELECTROPHORESIS ; MICROCHIP ELECTROPHORESIS ; STACKING INJECTION ; MICROFABRICATED DEVICES ; ZONE-ELECTROPHORESIS ; NEUTRAL ANALYTES ; ACID HERBICIDES
英文摘要We report a highly effective on-chip preconcentration method by combining field-amplified sample injection (FASI) and bovine serum albumin (BSA) sweeping for ultrasensitive detection of green fluorescent protein (GFP) on a simple cross-channel microchip device. With the formation of a stagnant sample/running buffer boundary by balancing the hydrodynamic flow and the electro-osmotic flow (EOF), GFP molecules can be continuously injected into the sample loading channel and stacked. We have also demonstrated that BSA is a very effective pseudo-stationary phase for sweeping concentration of proteins in comparison to the commonly used micelles. The combination of FASI and BSA sweeping yields a concentration factor of 3570 and a limit of detection of 8.4 pM for GFP. Using this method, we have separated GFP and GFP-insulin-like growth factor-I (GFP-IGF-I) fusion protein. The entire assay (GFP concentration, matrix elimination, and electrophoretic separation) can be completed within < 5 min. Furthermore, we have successfully applied this method for the detection of GFP expression of E. coli cells and the GFP content in single E. coli cells.
语种英语
WOS记录号WOS:000267609500032
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141521
专题中国科学院大连化学物理研究所
作者单位1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Univ Oklahoma, Dept Chem & Biochem, Norman, OK 73019 USA
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Pan, Qiong,Zhao, Meiping,Liu, Shaorong. Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein[J]. ANALYTICAL CHEMISTRY,2009,81(13):5333-5341.
APA Pan, Qiong,Zhao, Meiping,&Liu, Shaorong.(2009).Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein.ANALYTICAL CHEMISTRY,81(13),5333-5341.
MLA Pan, Qiong,et al."Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein".ANALYTICAL CHEMISTRY 81.13(2009):5333-5341.
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