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题名: Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein
作者: Pan, Qiong1;  Zhao, Meiping1;  Liu, Shaorong2
刊名: ANALYTICAL CHEMISTRY
发表日期: 2009-07-01
DOI: 10.1021/ac9007607
卷: 81, 期:13, 页:5333-5341
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: We report a highly effective on-chip preconcentration method by combining field-amplified sample injection (FASI) and bovine serum albumin (BSA) sweeping for ultrasensitive detection of green fluorescent protein (GFP) on a simple cross-channel microchip device. With the formation of a stagnant sample/running buffer boundary by balancing the hydrodynamic flow and the electro-osmotic flow (EOF), GFP molecules can be continuously injected into the sample loading channel and stacked. We have also demonstrated that BSA is a very effective pseudo-stationary phase for sweeping concentration of proteins in comparison to the commonly used micelles. The combination of FASI and BSA sweeping yields a concentration factor of 3570 and a limit of detection of 8.4 pM for GFP. Using this method, we have separated GFP and GFP-insulin-like growth factor-I (GFP-IGF-I) fusion protein. The entire assay (GFP concentration, matrix elimination, and electrophoretic separation) can be completed within < 5 min. Furthermore, we have successfully applied this method for the detection of GFP expression of E. coli cells and the GFP content in single E. coli cells.
关键词[WOS]: MICELLAR ELECTROKINETIC CHROMATOGRAPHY ;  AMPLIFIED SAMPLE INJECTION ;  ELECTROPHORESIS-MASS SPECTROMETRY ;  SYSTEM CAPILLARY-ELECTROPHORESIS ;  MICROCHIP ELECTROPHORESIS ;  STACKING INJECTION ;  MICROFABRICATED DEVICES ;  ZONE-ELECTROPHORESIS ;  NEUTRAL ANALYTES ;  ACID HERBICIDES
语种: 英语
WOS记录号: WOS:000267609500032
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141521
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Univ Oklahoma, Dept Chem & Biochem, Norman, OK 73019 USA

Recommended Citation:
Pan, Qiong,Zhao, Meiping,Liu, Shaorong. Combination of On-Chip Field Amplification and Bovine Serum Albumin Sweeping for Ultrasensitive Detection of Green Fluorescent Protein[J]. ANALYTICAL CHEMISTRY,2009,81(13):5333-5341.
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