DICP OpenIR
The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence
Wei, Chunying1; Wang, Lihua1; Jia, Guoqing2; Zhou, Jun2; Han, Gaoyi1; Li, Can2
关键词Binding Modes G-quadruplexes Interaction Porphyrin Spectroscopy
刊名BIOPHYSICAL CHEMISTRY
2009-07-01
DOI10.1016/j.bpc.2009.04.005
143期:1-2页:79-84
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Chemistry, Physical
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Chemistry
关键词[WOS]TIME-RESOLVED FLUORESCENCE ; STRUCTURAL BASIS ; LIGAND-BINDING ; TELOMERIC DNA ; GUANINE ; COMPLEXES ; STATE ; STABILIZATION ; SELECTIVITY ; TETRAPLEX
英文摘要Interactions of 5,10,15,20-Tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TMPyP4) and 5,10,15,20-Tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with the parallel four-stranded (TG(4)T)4 G-quadruplex DNA in 100 mM K+-containing buffer were studied using circular dichroism (CD) spectroscopy, visible absorption titration, and steady and time-resolved fluorescence spectroscopies. The results show that the binding stoichiometric ratios of both TMPyP4 and TPrPyP4 to (TG(4)T)4 are 3:1. Two types of independent and nonequivalent binding sites with the higher and lower binding affinities are confirmed, and the stronger and weaker binding constants are 9.44 x 10(7) and 6.94 x 10(5) M-1 for (TG(4)T)4-TMPyP4 complex, 7.86 x 10(7) and 6.35 x 10(5) M-1 for (TG(4)T)4-TPrPyP4 complex, respectively. For both TMPyP4-(TG(4)T)4 and TPrPyP4-(TG(4)T)4 complexes, one porphyrin molecule stacks on the one end of G-quadruplex with the higher binding affinity, another two porphyrins bind weakly to the two external grooves. The size of cation side arms around porphyrin core almost fails to affect the binding mode, stoichiometry and affinity of porphyrin to (TG(4)T)4 G-quadruplex in 100 mM K+-containing buffer. (C) 2009 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000267006000009
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141535
专题中国科学院大连化学物理研究所
作者单位1.Shanxi Univ, Key Lab Chem Biol & Mol Engn, Minist Educ, Inst Mol Sci, Taiyuan 030006, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Wei, Chunying,Wang, Lihua,Jia, Guoqing,et al. The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence[J]. BIOPHYSICAL CHEMISTRY,2009,143(1-2):79-84.
APA Wei, Chunying,Wang, Lihua,Jia, Guoqing,Zhou, Jun,Han, Gaoyi,&Li, Can.(2009).The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence.BIOPHYSICAL CHEMISTRY,143(1-2),79-84.
MLA Wei, Chunying,et al."The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence".BIOPHYSICAL CHEMISTRY 143.1-2(2009):79-84.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wei, Chunying]的文章
[Wang, Lihua]的文章
[Jia, Guoqing]的文章
百度学术
百度学术中相似的文章
[Wei, Chunying]的文章
[Wang, Lihua]的文章
[Jia, Guoqing]的文章
必应学术
必应学术中相似的文章
[Wei, Chunying]的文章
[Wang, Lihua]的文章
[Jia, Guoqing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。