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题名: The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence
作者: Wei, Chunying1;  Wang, Lihua1;  Jia, Guoqing2;  Zhou, Jun2;  Han, Gaoyi1;  Li, Can2
关键词: Binding modes ;  G-quadruplexes ;  Interaction ;  Porphyrin ;  Spectroscopy
刊名: BIOPHYSICAL CHEMISTRY
发表日期: 2009-07-01
DOI: 10.1016/j.bpc.2009.04.005
卷: 143, 期:1-2, 页:79-84
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ;  Biophysics ;  Chemistry, Physical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics ;  Chemistry
英文摘要: Interactions of 5,10,15,20-Tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TMPyP4) and 5,10,15,20-Tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with the parallel four-stranded (TG(4)T)4 G-quadruplex DNA in 100 mM K+-containing buffer were studied using circular dichroism (CD) spectroscopy, visible absorption titration, and steady and time-resolved fluorescence spectroscopies. The results show that the binding stoichiometric ratios of both TMPyP4 and TPrPyP4 to (TG(4)T)4 are 3:1. Two types of independent and nonequivalent binding sites with the higher and lower binding affinities are confirmed, and the stronger and weaker binding constants are 9.44 x 10(7) and 6.94 x 10(5) M-1 for (TG(4)T)4-TMPyP4 complex, 7.86 x 10(7) and 6.35 x 10(5) M-1 for (TG(4)T)4-TPrPyP4 complex, respectively. For both TMPyP4-(TG(4)T)4 and TPrPyP4-(TG(4)T)4 complexes, one porphyrin molecule stacks on the one end of G-quadruplex with the higher binding affinity, another two porphyrins bind weakly to the two external grooves. The size of cation side arms around porphyrin core almost fails to affect the binding mode, stoichiometry and affinity of porphyrin to (TG(4)T)4 G-quadruplex in 100 mM K+-containing buffer. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: TIME-RESOLVED FLUORESCENCE ;  STRUCTURAL BASIS ;  LIGAND-BINDING ;  TELOMERIC DNA ;  GUANINE ;  COMPLEXES ;  STATE ;  STABILIZATION ;  SELECTIVITY ;  TETRAPLEX
语种: 英语
WOS记录号: WOS:000267006000009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141535
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanxi Univ, Key Lab Chem Biol & Mol Engn, Minist Educ, Inst Mol Sci, Taiyuan 030006, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China

Recommended Citation:
Wei, Chunying,Wang, Lihua,Jia, Guoqing,et al. The binding mode of porphyrins with cation side arms to (TG(4)T)4 G-quadruplex: Spectroscopic evidence[J]. BIOPHYSICAL CHEMISTRY,2009,143(1-2):79-84.
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