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Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail
Hu, Ying1,2; Luan, Hongwei1,2; Liu, Huixin1,2; Ge, Guangbo1,2; Zhou, Kun1,2; Liu, Yong1; Yang, Ling1
关键词Beta-d-glycosidase Disaccharide Synthesis Broad Acceptor Specificity Transfer Efficiency China White Jade Snail
刊名BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
2009-03-01
DOI10.1271/bbb.80724
73期:3页:671-676
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
关键词[WOS]BIFIDOBACTERIUM BREVE CLB ; ENZYMATIC-SYNTHESIS ; THERMUS-THERMOPHILUS ; ASPERGILLUS-ORYZAE ; ESCHERICHIA-COLI ; D-GLUCOSIDASE ; TRANSGLYCOSYLATION ; OLIGOSACCHARIDES ; PURIFICATION ; ALPHA
英文摘要The acceptor specificity and transfer potential of a beta-D-glycosidase (G I), which had been purified from the China white jade snail, were further investigated by using various sugars as acceptors. G I had broad monosaccharide acceptor specificity for its transglycosylation activity. More specifically, it efficiently catalyzed the transfer of the beta-D-fucosyl, beta-D-glucosyl or beta-D-galactosyl moiety from the corresponding p-nitrophenyl-beta-D-glycopyranosides to various monosaccharides. The transfucosylation efficiency of G I was studied by using p-nitrophenyl-beta-D-fucopyranoside (pNPFuc) as the donor and glucose and xylose as the acceptors. The yields under conditions of non-initial velocity were 88% for glucose and 93% for xylose. The transfer product with glucose as the acceptor was isolated and identified as beta-fucosyl-1,6-glucose by an NMR analysis. The data from these analyses indicate that G I had broad acceptor specificity and high efficiency for transglycosylation. These uncommon properties of G I could make it a valuable biocatalyst for the synthesis of various disaccharides.
语种英语
WOS记录号WOS:000264904900031
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141540
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
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Hu, Ying,Luan, Hongwei,Liu, Huixin,et al. Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2009,73(3):671-676.
APA Hu, Ying.,Luan, Hongwei.,Liu, Huixin.,Ge, Guangbo.,Zhou, Kun.,...&Yang, Ling.(2009).Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,73(3),671-676.
MLA Hu, Ying,et al."Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73.3(2009):671-676.
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