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题名: Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail
作者: Hu, Ying1, 2;  Luan, Hongwei1, 2;  Liu, Huixin1, 2;  Ge, Guangbo1, 2;  Zhou, Kun1, 2;  Liu, Yong1;  Yang, Ling1
关键词: beta-D-glycosidase ;  disaccharide synthesis ;  broad acceptor specificity ;  transfer efficiency ;  China white jade snail
刊名: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
发表日期: 2009-03-01
DOI: 10.1271/bbb.80724
卷: 73, 期:3, 页:671-676
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Chemistry, Applied ;  Food Science & Technology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Food Science & Technology
英文摘要: The acceptor specificity and transfer potential of a beta-D-glycosidase (G I), which had been purified from the China white jade snail, were further investigated by using various sugars as acceptors. G I had broad monosaccharide acceptor specificity for its transglycosylation activity. More specifically, it efficiently catalyzed the transfer of the beta-D-fucosyl, beta-D-glucosyl or beta-D-galactosyl moiety from the corresponding p-nitrophenyl-beta-D-glycopyranosides to various monosaccharides. The transfucosylation efficiency of G I was studied by using p-nitrophenyl-beta-D-fucopyranoside (pNPFuc) as the donor and glucose and xylose as the acceptors. The yields under conditions of non-initial velocity were 88% for glucose and 93% for xylose. The transfer product with glucose as the acceptor was isolated and identified as beta-fucosyl-1,6-glucose by an NMR analysis. The data from these analyses indicate that G I had broad acceptor specificity and high efficiency for transglycosylation. These uncommon properties of G I could make it a valuable biocatalyst for the synthesis of various disaccharides.
关键词[WOS]: BIFIDOBACTERIUM BREVE CLB ;  ENZYMATIC-SYNTHESIS ;  THERMUS-THERMOPHILUS ;  ASPERGILLUS-ORYZAE ;  ESCHERICHIA-COLI ;  D-GLUCOSIDASE ;  TRANSGLYCOSYLATION ;  OLIGOSACCHARIDES ;  PURIFICATION ;  ALPHA
语种: 英语
WOS记录号: WOS:000264904900031
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141540
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China

Recommended Citation:
Hu, Ying,Luan, Hongwei,Liu, Huixin,et al. Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2009,73(3):671-676.
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