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Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail
Hu, Ying1,2; Luan, Hongwei1,2; Liu, Huixin1,2; Ge, Guangbo1,2; Zhou, Kun1,2; Liu, Yong1; Yang, Ling1
KeywordBeta-d-glycosidase Disaccharide Synthesis Broad Acceptor Specificity Transfer Efficiency China White Jade Snail
Source PublicationBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
2009-03-01
DOI10.1271/bbb.80724
Volume73Issue:3Pages:671-676
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry, Applied ; Food Science & Technology
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Chemistry ; Food Science & Technology
WOS KeywordBIFIDOBACTERIUM BREVE CLB ; ENZYMATIC-SYNTHESIS ; THERMUS-THERMOPHILUS ; ASPERGILLUS-ORYZAE ; ESCHERICHIA-COLI ; D-GLUCOSIDASE ; TRANSGLYCOSYLATION ; OLIGOSACCHARIDES ; PURIFICATION ; ALPHA
AbstractThe acceptor specificity and transfer potential of a beta-D-glycosidase (G I), which had been purified from the China white jade snail, were further investigated by using various sugars as acceptors. G I had broad monosaccharide acceptor specificity for its transglycosylation activity. More specifically, it efficiently catalyzed the transfer of the beta-D-fucosyl, beta-D-glucosyl or beta-D-galactosyl moiety from the corresponding p-nitrophenyl-beta-D-glycopyranosides to various monosaccharides. The transfucosylation efficiency of G I was studied by using p-nitrophenyl-beta-D-fucopyranoside (pNPFuc) as the donor and glucose and xylose as the acceptors. The yields under conditions of non-initial velocity were 88% for glucose and 93% for xylose. The transfer product with glucose as the acceptor was isolated and identified as beta-fucosyl-1,6-glucose by an NMR analysis. The data from these analyses indicate that G I had broad acceptor specificity and high efficiency for transglycosylation. These uncommon properties of G I could make it a valuable biocatalyst for the synthesis of various disaccharides.
Language英语
WOS IDWOS:000264904900031
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141540
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Hu, Ying,Luan, Hongwei,Liu, Huixin,et al. Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,2009,73(3):671-676.
APA Hu, Ying.,Luan, Hongwei.,Liu, Huixin.,Ge, Guangbo.,Zhou, Kun.,...&Yang, Ling.(2009).Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail.BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,73(3),671-676.
MLA Hu, Ying,et al."Acceptor Specificity and Transfer Efficiency of a beta-Glycosidase from the China White Jade Snail".BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 73.3(2009):671-676.
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