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题名: A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis
作者: Zhang, Yu1, 2;  Yu, Hao1;  Qin, Jianhua1;  Lin, Bingcheng1
关键词: biochemistry ;  biocomputing ;  Boolean algebra ;  cancer ;  DNA ;  genomics ;  lab-on-a-chip ;  microfluidics
刊名: BIOMICROFLUIDICS
发表日期: 2009-10-01
DOI: 10.1063/1.3259628
卷: 3, 期:4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biophysics ;  Nanoscience & Nanotechnology ;  Physics, Fluids & Plasmas
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics ;  Science & Technology - Other Topics ;  Physics
英文摘要: Boolean logic performs a logical operation on one or more logic input and produces a single logic output. Here, we describe a microfluidic DNA computing processor performing Boolean logic operations for gene expression analysis and gene drug synthesis. Multiple cancer-related genes were used as input molecules. Their expression levels were identified by interacting with the computing related DNA strands, which were designed according to the sequences of cancer-related genes and the suicide gene. When all the expressions of the cancer-related genes fit in with the diagnostic criteria, positive diagnosis would be confirmed and then a complete suicide gene (gene drug) could be synthesized as an output molecule. Microfluidic chip was employed as an effective platform to realize the computing process by integrating multistep biochemical reactions involving hybridization, displacement, denaturalization, and ligation. By combining the specific design of the computing related molecules and the integrated functions of the microfluidics, the microfluidic DNA computing processor is able to analyze the multiple gene expressions simultaneously and realize the corresponding gene drug synthesis with simplicity and fast speed, which demonstrates the potential of this platform for DNA computing in biomedical applications.
关键词[WOS]: HYDROGEL PLUGS ;  BREAST-CANCER ;  CHANNELS ;  SYSTEM ;  CHIP ;  POLYMORPHISM ;  THERAPY ;  MACHINE ;  ASSAY
语种: 英语
WOS记录号: WOS:000273217500005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141546
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Yu,Yu, Hao,Qin, Jianhua,et al. A microfluidic DNA computing processor for gene expression analysis and gene drug synthesis[J]. BIOMICROFLUIDICS,2009,3(4).
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