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题名: "Click dipeptide": A novel stationary phase applied in two-dimensional liquid chromatography
作者: Xue, Meiyun1;  Huang, Hongxue1;  Ke, Yanxiong1;  Chu, Changhu1;  Jin, Yu1;  Liang, Xinmiao1, 2
关键词: Click dipeptide stationary phase ;  alpha-Azido L-phenylglycine dipeptide ;  Orthogonality ;  2D-RP/RPLC ;  Complex sample
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-12-04
DOI: 10.1016/j.chroma.2009.10.019
卷: 1216, 期:49, 页:8623-8629
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: 2D-HPLC is an important technique for the separation of complex samples. Developing new types of stationary phases is of great interest to construct 2D-LC systems with high orthogonality. In this study, a novel stationary phase-Click dipeptide (L-phenylglycine dipeptide) was prepared by immobilization of alpha-azido L-phenylglycine dipeptide on alkyne-silica via click chemistry. In the preparation of this new material, an efficient, inexpensive and shelf-stable diazo transfer reagent (imidazole-1-sulfonyl azide hydrochloride) was utilized to transfer the amino group of L-phenylglycine to corresponding azido group under mild conditions. The Click dipeptide thus prepared was confirmed by FT-IR, solid state CP/MAS (13)C NMR and elemental analysis. The Click dipeptide packing showed high orthogonality with C18, which reached 63.5%. An off-line 2D-RP/RPLC system was developed to analyze a traditional Chinese medicine (TCM)-Rheum Palmatum L. The results showed high orthogonality between Click dipeptide and C18 as well as great separating power in the practical separation of complex samples. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: ISOLATED PROTEIN-FRAGMENTS ;  REVERSED-PHASE ;  OLIGO(ETHYLENE GLYCOL) ;  HPLC SYSTEM ;  SILICA-GEL ;  SEPARATION ;  CHEMISTRY ;  PEPTIDES ;  EFFICIENT ;  COLUMNS
语种: 英语
WOS记录号: WOS:000272089200008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141551
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.E China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Xue, Meiyun,Huang, Hongxue,Ke, Yanxiong,et al. "Click dipeptide": A novel stationary phase applied in two-dimensional liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(49):8623-8629.
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