中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry
作者: Luo Fu-Wen2;  Tao Ding-Yin1;  Zhao Peng2;  Zhang Li-Hua1;  Jia Yu-Jie2;  Zhang Yu-Kui1
关键词: Stomach cancer tissue ;  proteomics ;  two-dimensional high performance liquid chromatography-mass spectrometry
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2009-10-01
卷: 37, 期:10, 页:1403-1408
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A two-dimensional high performance liquid chromatographic separation platform based on strong cation exchange (SCX) chromatography and reversed phase ( RP) chromatography was developed. Peptides were first separated by a SCX column under stepwise gradient of 0, 20, 200,300, 500, 1000 and 2000 mmol/L NH4AC buffer. After each fraction was collected and desalted by a RP trap column, further separation was implemented on a RPLC column under linear gradient elution. All the components were identified by mass spectrometry after 2D separation. The platform was applied to the analysis of proteins in stomach cancer tissue. The number of identified proteins were 537 and 506 for healthy and tumor tissue respectively. According to the physical characters of proteins identified, the distributions of these identified proteins were obtained and the difference between healthy and tumor tissue was found. The retrieval results were compared to screen out diagnostic proteins related to pathological changes of tumor tissues.
语种: 英语
WOS记录号: WOS:000271686300001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141552
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Dept Gen Surg 2, Dalian 116027, Peoples R China

Recommended Citation:
Luo Fu-Wen,Tao Ding-Yin,Zhao Peng,et al. Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(10):1403-1408.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Luo Fu-Wen]'s Articles
 [Tao Ding-Yin]'s Articles
 [Zhao Peng]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Luo Fu-Wen]‘s Articles
 [Tao Ding-Yin]‘s Articles
 [Zhao Peng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace